SNP sites related to number of pig nipples and detection method and application thereof

A locus and nipple technology, applied in the field of molecular genetics, can solve the problems of inability to achieve large-scale sequencing, limited labeling density, limited application, etc., and achieve excellent economic value, increase the number of nipples, and low cost.

Active Publication Date: 2020-01-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the simplified genome has limited its application in fine mapping due to the limited marker density.
Genome-wide association analysis involves all the variations on the genome, but the cost is high and large sample size sequencing cannot be achieved

Method used

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  • SNP sites related to number of pig nipples and detection method and application thereof
  • SNP sites related to number of pig nipples and detection method and application thereof
  • SNP sites related to number of pig nipples and detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Genome-wide association analysis of pig teat number traits

[0034] The performance records of 33,960 individuals (12,987 boars and 20,973 sows) born between August 2007 and January 2016 were collected for this study.

[0035] 2. Test method

[0036] 2.1 Teat number phenotype: the number of left teats, right teats and total teats of each pig were recorded within one week after the individual was born.

[0037] 2.2 Tn5 transposase library construction:

[0038] (1) The genome was uniformly diluted to 40ng / μl, and 50ng of each sample was taken as the initial amount. Tn5 proenzyme and specific Tn5ME-A / Tn5Merev and Tn5ME-B / Tn5MErev linkers were embedded at 72°C for 2h to obtain Tn5 working enzyme with cut-paste activity. The linker structure is the same as the Tn5ME-A, Tn5ME-B, and Tn5Merev structures disclosed in the document "Tn5 transposase and tagmentation procedures formatively scaled sequencing projects".

[0039] (2) Dilute Tn5 working enzyme to 16.5ng / ...

Embodiment 2

[0051] Example 2: Breeding application of SNP locus (SSC7:97570528) in pig teat number traits

[0052] 1. Test material

[0053] A population of Duroc purebred pigs with the teat number trait.

[0054] 2. Test method

[0055] Association analysis of missense mutations and teat number traits: 7 missense mutation loci were genotyped, and association analysis was performed between genotypes and teat number traits of Duroc pigs.

[0056] Table 2 shows the results of the association analysis between the genotypes of the 7 missense mutation sites and the number of papillae traits. SSC7:97570528 site, AA is the dominant genotype. SSC7: 97574214 site, CC is the dominant genotype; SSC7: 98073927 site, AA is the dominant genotype; SSC7: 98074438 site, TT is the dominant genotype; SSC7: 98074524 site, TT is the dominant genotype; SSC7: At site 98074744, CC is the dominant genotype; at site SSC7:98101170, GG is the dominant genotype.

[0057] Table 2 - Least squares mean and standard...

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Abstract

The invention relates to SNP sites related to the number of pig nipples and a detection method and application thereof. The SNP sites are located at a chr7:97570528 of a genomic version Ensembl Sscrofa 11.1, and alleles of the sites are A and G. According to the SNP sites related to the number of the pig nipples and the detection method and application thereof, Tn5 transposase is used for establishing a library, low-depth sequencing is carried out on a core population of 3,616 Duroc pigs with important economic performance records, and high-quality whole-genome resequencing data are obtained through padding. Through GWAS analysis of the characteristics of the number of the pig nipples, seven SNP sites which significantly affect the number of the pig nipples are detected, and a newly discovered SNP site SSC7: 97570528 with the most significant effect is included. The SNP sites can be used as molecular markers for the breeding of dominant populations of the number of the pig nipples.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a SNP site related to the number of pig teats and its detection method and application, specifically a newly discovered missense mutation related to the number of pig teats and its application. Background technique [0002] my country is the largest pig producing and consuming country, and the market demand for pork production and quality is increasing day by day. Increasing pork production and improving pork carcass quality have become the work that breeding scientists have been exploring for a long time. Early breeding efforts were mainly focused on phenotypic selection in pigs. With the continuous advancement of genomic work and the extensive development of genetic markers, molecular selection is gradually becoming a reliable and effective selection method. [0003] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) marker is the third-generation molecular m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11G16B20/30G16B30/10
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172G16B20/30G16B30/10
Inventor 胡晓湘郭晓莉王宇哲杨瑞飞朱迪李宁
Owner CHINA AGRI UNIV
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