Combination of five cytokines as ionizing radiation injury biomarker

A biomarker and cytokine technology, applied in the field of ionizing radiation damage biomarkers, can solve the problems of high cost, cumbersome experiments, and long time consumption

Active Publication Date: 2020-01-10
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the problems of traditional biological methods of radiation damage monitoring and dose estimation, such as time-consuming, complicated experiments, high cost, and narrow response dose measurement range, the present invention provides a method for detecting ionizing radiation damage using five cytokine combinations as biomarkers. Methods

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  • Combination of five cytokines as ionizing radiation injury biomarker
  • Combination of five cytokines as ionizing radiation injury biomarker
  • Combination of five cytokines as ionizing radiation injury biomarker

Examples

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Embodiment 1

[0098] Example 1. Five cytokines as biomarkers for ionizing radiation damage

[0099] The present invention is based on the high sensitivity and high throughput of the MSD instrument (MSD: hypersensitive factor electrochemiluminescence analyzer), and is sensitive to serum and stress responses to experimental mice after radiation. 29 kinds of cytokines (including inflammatory cytokines, pro-inflammatory cytokines, immune factors, specifically IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL -10, IL-12p70, IL-15, IL-16, IL-17A, IL-17A / F, IL-17C, IL-17E / IL-25, IL-17F, IL-21, IL-22, IL -23, IL-27p28 / IL-30, IL-31, IL-33, IP-10, KC / GRO, MCP-1, MIP-1α, MIP-2, MIP-3α, TNF-α) for detection, Through experimental analysis and comparative screening, it was found that among the five cytokines, the expression levels of IL-5 and IL-6 had a certain correlation with the exposure dose after irradiation, and the expression level of IL-33 increased after irradiation , and the expression levels of...

Embodiment 2

[0185] Example 2, Example of Estimating Radiation Dose Using IL-5 and IL-6 Factors

[0186] 1. Preparation of samples to be tested:

[0187] The X-rays produced by the Faxitron R650 X-ray instrument are used as the representative of low-LET ionizing radiation, and the carbon ion beam produced by the biological irradiation terminal of Lanzhou Heavy Ion Accelerator Cooling Storage Ring (HIRFL-CSR) is used as the representative of high-LET ionizing radiation. Kunming mice, experimental animals with good growth status at 6-8 weeks, were subjected to whole-body irradiation. The irradiation doses were 0.5Gy, 2Gy, 4Gy and 8Gy, 15 rats in each group; blood was collected by eyeball at 1 hour and 7 days after irradiation. Centrifuge at 3000 rpm at 4°C for 10 minutes, and absorb the upper serum for later use. The control group was unirradiated Kunming mice, experimental animals with good growth status for 6-8 weeks.

[0188] 2. Prepare buffer solution, coating solution, standard sampl...

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Abstract

The invention discloses five cytokines as ionizing radiation injury biomarkers. The invention provides an application of a combination of five cytokines IL-5, IL-6, IL-10, IL-15 and IL-33 as a biomarker in monitoring animal individual harm or crowd harm caused by radiation leakage. According to the method, a serum sample of an irradiated victim is taken as a detection object, and blood can be collected at any time after irradiation for detection and evaluation, thereby achieving the timely evaluation; based on the advantages of MSD equipment, the detection with low sample size requirement, high flux and high sensitivity can be realized. According to the method, the required sample amount for detection is small, five strictly analyzed and screened radiation damage biomarkers are detected ata time, the low-cost, rapid and efficient detection function can be achieved, and the service radiation hazards are evaluated in time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the combination of five cytokines as ionizing radiation damage biomarkers. Background technique [0002] With the wide application of nuclear energy and nuclear medicine technology, the impact of radiation on the environment and human health has attracted more and more attention. Assessing radiation hazards, diagnosing and treating various types of radiation injuries requires active development of new methods and technologies for rapid assessment of biological exposure doses. Traditional biological methods for estimating radiation dose include chromosomal aberration analysis, precocious condensed chromosome fragment analysis, micronucleus analysis, somatic cell mutation analysis, etc. Recently, Columbia University established a high-throughput method-RABiT (Rapid Automated Biodosimetry Tool) to detect the DNA damage marker γ-H2AX. However, these methods have certain disadvantages. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6869G01N33/6893G01N2800/60G01N2800/40G01N2333/5409G01N2333/5412G01N2333/5428G01N2333/5443G01N2333/54
Inventor 胡步荣贾蓉陈亚雄杜亚蓉吴庆丰李占奎
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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