Method and device for preparing hydrogen-rich water by utilizing photosynthetic microalgae
A technology of photosynthetic microalgae and hydrogen-rich water, applied in the direction of adding substances to water/sewage treatment, etc., can solve hidden dangers, hydrogen waste safety and other problems, and achieve the effect of high hydrogen content
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[0048] figure 1 It is a schematic diagram of the device for preparing hydrogen-rich water of the present invention.
[0049] The device includes a microalgae photosynthetic hydrogen production system, a drainage and air collection system and a hydrogen-rich water collection system. The microalgae photosynthetic hydrogen production system consists of a 2L microalgae culture bottle, a set of LED lights (8 in total), a magnetic stirrer, two solar panels and two batteries; A sterile glass bottle of saline; the hydrogen-rich water collection system consists of four vacuum sterile bags connected in series. The three systems are connected by sterile catheters with controllable valves.
[0050] The working principle and operation of this device are very simple. The solar panel converts solar energy into electrical energy and stores it in the battery. The battery supplies power to the LED lights and magnetic stirrer to support the microalgae to continuously generate hydrogen. After t...
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[0052] 1. Materials and methods
[0053] 1.1 Materials
[0054] 1.1.1 Plant material: Chlamydomonas reinhardtii (Chlamydomonas reinhardtii), algae species CC124 is the product of Duke University algae species Chlamydomonas reinhardtii (Chlamy center).
[0055] 1.1.2 Medium
[0056] 1. TAP medium (g / L): NH 4 Cl 0.4; MgSO 4 ·7H 2 O 0.1; CaCl 2 2H 2 O 0.05; K 2 HPO 4 0.108; KH 2 PO 4 0.056; Trisbase 2.423; Hutner's trace element 1mL, glacial acetic acid 1ml (product of Beijing Chemical Reagent Company).
[0057] 2. Sulfur-deficient TAP culture solution (g / L): The sulfide in the TAP culture solution was replaced with the corresponding chloride (product of Beijing Chemical Reagent Co., Ltd.) in an equimolar manner.
[0058] 3. Solid TAP medium: add 1.5% agar powder to the TAP medium.
[0059] 4. Hutner’s trace element (g / L): (product of sigma company)
[0060]
[0061] 1.1.3 Other reagents
[0062] Sterile physiological saline: 0.9% NaCl solution, after high tempe...
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