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Method for analyzing sucrose ester in tobacco through multi-dimensional liquid chromatography-mass spectrometry

A multi-dimensional liquid chromatography and liquid chromatography technology, applied in the field of chemical composition analysis in tobacco, can solve problems such as complex pretreatment and complicated pretreatment steps

Active Publication Date: 2019-12-31
SHANGHAI TOBACCO GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GC-MS method requires derivatization treatment, complicated pretreatment
The LC-MS method does not require derivatization, but still requires complex pretreatment steps, such as repeated liquid-liquid extraction, etc.

Method used

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  • Method for analyzing sucrose ester in tobacco through multi-dimensional liquid chromatography-mass spectrometry
  • Method for analyzing sucrose ester in tobacco through multi-dimensional liquid chromatography-mass spectrometry
  • Method for analyzing sucrose ester in tobacco through multi-dimensional liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0160] 1. Sample pretreatment

[0161] Take 0.2g tobacco sample, add 0.1mL internal standard sample and 5mL acetonitrile for ultrasonic extraction for 20min, centrifuge at 2000r / min for 10min, pass through a 0.22μm organic phase filter to obtain a sample solution. The internal standard sample was sucrose monocaprate.

[0162] 2. Determination

[0163] The sample solution is measured by two-dimensional liquid chromatography mass spectrometry (LC-LC-MS), the sucrose ester component and the internal standard sample in the sample solution are qualitatively carried out by mass spectrometry, and the internal standard sample in the sample solution is analyzed by the standard curve method Quantification is carried out, and then the relative content of the sucrose ester components similar in structure to the internal standard sample determined quantitatively in the sample solution is determined.

[0164] Among them, two-dimensional liquid chromatography-mass spectrometry (LC-LC-MS) i...

Embodiment 2

[0179] The oriental tobacco with higher sucrose ester content was used to conduct the repeatability investigation of the method, the pretreatment of step 1 in the above example 1 was carried out, and the LC-LC-MS analysis of step 2 in the above example 1 was carried out, and 6 types of different sucrose were found For ester and SOE, the repeatability of the method is about 10%, which meets the needs of detection. The results are shown in Table 6.

[0180] Table 6 Method Repeatability Investigation of Sucrose Esters

[0181]

Embodiment 3

[0183] Select different types of tobacco samples such as flue-cured green tobacco leaves, flue-cured tobacco, and oriental tobacco after roasting, and measure the content of sucrose esters, perform the pretreatment of step 1 in the above-mentioned embodiment 1, and carry out the step 2 in the above-mentioned embodiment 1 LC-LC-MS analysis, the specific measurement results are shown in Table 7. It can be seen from Table 7 that compared with post-cured flue-cured tobacco, the content of sucrose esters in oriental tobacco is generally higher, especially STE III, STE IV, and STE V. It is possible that the contribution of such substances to the aroma components is relatively large. Compared with flue-cured green tobacco and flue-cured tobacco, the content of STE II components is higher, and its relative content in the total sucrose ester is also higher.

[0184] Relative content of sucrose esters in table 7 actual samples

[0185]

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Abstract

The invention provides a method for analyzing sucrose ester in tobacco through multi-dimensional liquid chromatography-mass spectrometry. The method comprises the steps of adding an internal standardsample into a tobacco sample, adding a solvent, extracting, centrifuging and filtering to obtain a sample solution; and then, measuring the sample solution by adopting a two-dimensional liquid chromatography-mass spectrometry combination method, qualitatively determining the sucrose ester component and the internal standard sample in the sample solution by mass spectrometry, quantitatively determining the internal standard sample in the sample solution through a standard curve method, and determining the relative content of the sucrose ester component with the structure similar to that of theinternal standard sample determined quantitatively in the sample solution. The invention further provides a multi-dimensional liquid chromatography-mass spectrometry combined analysis system and a using method thereof. The method for analyzing the sucrose ester in the tobacco by the multi-dimensional liquid chromatography-mass spectrometry can effectively remove interfering substances which influence the quantification of the sucrose ester in the tobacco, has the characteristics of being high in accuracy, good in reproducibility and low in detection limit, the analysis can be automatically accomplished through an instrument, and the method is suitable for the batch detection of the tobacco sample.

Description

technical field [0001] The invention belongs to the technical field of chemical component analysis in tobacco, and relates to a method for analyzing sucrose esters in tobacco by multi-dimensional liquid chromatography-mass spectrometry, in particular to a method for analyzing sucrose esters in tobacco by using multi-dimensional liquid chromatography-mass spectrometry and heart cutting method of relative amounts of ingredients. Background technique [0002] Sucrose ester (SE) is an important class of flavor precursors in tobacco, which mainly exists in the waxy layer on the surface of tobacco leaves. The sucrose ester components in tobacco mainly include 6 kinds, namely sucrose ester SE I, SE II, SE III , SE IV, SE V, and SEVI. For different types of tobacco leaves, the content of SE was quite different. Among them, the SE content in oriental tobacco was higher, and that in Burley and flue-cured tobacco samples was lower. SE itself has no special smell, but it will burn an...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88G01N30/74
CPCG01N30/06G01N30/88G01N30/74
Inventor 陈敏董惠忠刘百战吴达
Owner SHANGHAI TOBACCO GRP CO LTD
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