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Method for quickly and preliminarily screening excellent tea tree resource based on contained contents

A tea tree, an excellent technology, applied in the field of rapid initial screening of excellent tea tree resources, can solve the problems of inability to screen out excellent germplasm, use a large amount of organic solvent, and take a long time, and achieve shortened preparation time, simple method, and less materials Effect

Inactive Publication Date: 2014-03-05
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method steps are cumbersome, not only takes a lot of time, but also consumes a large amount of organic solvent, the extraction efficiency is low, and the amount of material required is large, and the sample needs to be dried.
It can be seen that the classic chemical screening method is not suitable for screening out excellent germplasm from a large number of tea tree resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Taking wild tea resources in Huangjin Village, Hulu Town, Baojing County, Xiangxi, Hunan Province as the investigation object, sampling was taken on April 6, 2010, and a total of 1000 samples were collected. The sampling method is to take a sample from each wild tea tree, cut the first unfolded leaf on the bud head farthest from the ground, put it into a 2mL plastic centrifuge tube without damage, close the lid, and mark the source of the sample. After the sampling is completed, the collected samples are brought back to the laboratory. After accurate weighing, freeze in liquid nitrogen for 30 s, take out the centrifuge tube, open the lid, quickly smash the leaves with a glass rod, add 1.5 mL of analytical pure methanol to the centrifuge tube, close the centrifuge tube cover tightly, and vortex for 30 s , incubate at 60°C for 10 min, vortex for 1 min, cool to room temperature, centrifuge at 12,000 rpm / min for 5 min, take the supernatant, and analyze the content of catech...

Embodiment 2

[0022] Taking wild tea tree resources in Hulu Village, Hulu Town, Hulu Town, Baojing County, Xiangxi, Hunan Province as the investigation object, sampling was taken on April 10, 2011, and a total of 600 samples were collected. The sampling method is to take a sample from each wild tea tree, cut the first unfolded leaf on the bud head farthest from the ground, put it into a 2mL plastic centrifuge tube without damage, close the lid, and mark the source of the sample. After the sampling is completed, the collected samples are brought back to the laboratory. After accurate weighing, freeze in liquid nitrogen for 60 seconds, take out the centrifuge tube, open the lid, quickly smash the leaves with a glass rod, add 1.5 mL methanol (analytical grade) to the centrifuge tube, cover the centrifuge tube tightly, and vortex For 30s, incubate at 70°C for 5min, vortex for 2min, cool to room temperature, centrifuge at 14000rpm / min for 2min, take the supernatant, and analyze the content of ca...

Embodiment 3

[0024]Taking wild tea tree resources in Gaosha Town, Dongkou County, Shaoyang City, Hunan Province as the investigation object, samples were taken on April 18, 2012, and a total of 500 samples were collected. The sampling method is to take a sample from each wild tea tree, cut the first unfolded leaf on the bud head farthest from the ground, put it into a 2mL plastic centrifuge tube without damage, close the lid, and mark the source of the sample. After the sampling is completed, the collected samples are brought back to the laboratory. After accurate weighing, freeze in liquid nitrogen for 45 seconds, take out the centrifuge tube, open the cover, quickly crush the leaves with a glass rod, add 1.5 mL of analytical pure methanol to the centrifuge tube, cover the centrifuge tube tightly, vortex for 30 seconds, Incubate at 65°C for 8 minutes, vortex for 90s, cool to room temperature, centrifuge at 13000rpm / min for 3 minutes, take the supernatant, and analyze the content of catech...

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Abstract

The invention discloses a method for quickly and preliminarily screening excellent tea tree resources based on contained contents. The method comprises the following steps: taking first expanded leaves of tea tree buds with consistent growth conditions and growth vigor, performing freezing treatment to the leaves by liquid nitrogen, crushing the leaves, using methanol to quickly and directly extract the leaves, centrifuging the leaves, taking supernatant, utilizing a high performance liquid chromatography device to detect the contained contents in the tea leaves, comparing the contents of the contained ingredients, preliminarily screening the excellent tea tree genetic resources, and based on the preliminary screening, adopting the known method to further screen the preliminarily screened excellent tea tree genetic resources. The method is simple and convenient, low in the device requirements, low in the reagent dosage, simplifies the pretreatment procedures of the test materials, greatly reduces the labor intensity, reduces the determination cost and time, and can be used for high throughput analysis.

Description

technical field [0001] The invention belongs to the technical field of economic crop germplasm innovation, and in particular relates to a method for rapid primary screening of excellent tea tree resources based on contained components. Background technique [0002] Tea is an important economic crop and a traditional export product in China. China's tea plantation area and annual output both rank first in the world. As the origin of tea trees, China's tea regions span the warm temperate zone, subtropics and marginal tropics, and are rich in ecological resources and tea tree germplasm resources, which are a huge treasure of tea tree genetic variation. According to incomplete statistics, more than 3,000 tea tree germplasm resources have been collected and preserved in various tea-producing provinces / regions across the country, and the number of tea tree resources that have not yet been put into nurseries is even greater. These excellent tea tree germplasm resources are the mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 刘硕谦李娟黄建安刘仲华李银花林海燕田娜
Owner HUNAN AGRICULTURAL UNIV
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