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Multi-element paper-based sliding microfluidic chip based on cerium metal organic framework

A metal-organic framework, microfluidic chip technology, applied in laboratory containers, material analysis by observing the effect on chemical indicators, laboratory utensils, etc., can solve problems such as uneven color development, and achieve processing. Simple steps, low cost and high thermal stability

Active Publication Date: 2019-12-31
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inhomogeneity of color development is a major problem faced by the current colorimetric detection method, especially the situation involving liquid flow during chip use
At the same time, for the detection of serum samples, after drying, the serum will form a light yellow film on the surface of the filter paper, which will cover the color development of the detection area, so it is a major bottleneck in the detection of serum samples by color development methods

Method used

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  • Multi-element paper-based sliding microfluidic chip based on cerium metal organic framework
  • Multi-element paper-based sliding microfluidic chip based on cerium metal organic framework
  • Multi-element paper-based sliding microfluidic chip based on cerium metal organic framework

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Structure of paper-based sliding microfluidic chip

[0049] Such as Figure 1-3 As shown, a multi-component paper-based sliding microfluidic chip for visually and quantitatively detecting the content of glucose and uric acid, the substrate is porous filter paper, including the upper reaction chip a and the lower color development chip b. The upper layer reaction chip a and the lower layer color development chip b have the same shape and size, and both include a rectangular main body area and side folding wings, and the main body area is divided into a hydrophilic area and a hydrophobic area; The upper and lower chips are assembled and fixed by the origami method, and slides are provided for the sliding of the upper and lower chips; the hydrophilic region includes the reaction region and the color region, maintaining the porous nature of the filter paper, and the liquid can penetrate and diffuse in this region; The hydrophobic area is the other part of the main body exc...

Embodiment 2

[0086] Preparation method of paper-based sliding microfluidic chip for quantitative detection of glucose content

[0087] Include the following steps:

[0088] (1) Use Adobe Illustrator to design the upper and lower layer patterns of the chip;

[0089] (2) Use a Xerox Phaser 8570DN wax spray printer to print the upper and lower layer patterns on A4-sized porous medium-speed filter paper;

[0090] (3) Cut out the printed upper and lower layer patterns;

[0091] (4) Heat the cut patterned filter paper on a heating platform at 160°C for 2 minutes to cool it down;

[0092] (5) 60 μL of BSA (30 μM) and EDC (100 mM) were dissolved in PBS buffer (pH=5.4), added dropwise to the hydrophilic area of ​​the upper reaction chip, and dried at room temperature; 60 μL of BSA (30 μM), glucose Oxidase (16 μM) or urate oxidase and EDC (100 mM) were dissolved in PBS buffer (pH=5.4), added dropwise to the hydrophilic area of ​​the upper reaction chip, and dried at room temperature for use;

[00...

Embodiment 3

[0096] Method for detection of glucose and uric acid by paper-based sliding microfluidic chip

[0097] S1. Prepare a multi-component paper-based sliding microfluidic chip: take a multi-component paper-based sliding microfluidic chip for visual and quantitative detection of glucose and uric acid content in the sample;

[0098] S2. Add glucose (uric acid) standard solution dropwise to the reaction area of ​​the upper reaction chip, and incubate at 37°C;

[0099] S3. Slide the upper and lower paper chips to connect to the hydrophilic channel, and the hydrogen peroxide generated on the upper sheet migrates to the color development area of ​​the lower layer due to the gravity of the solution and the wicking effect of the filter paper, and completes the color development reaction and is dried;

[0100] S4. Flip the dried chip over, use a mobile phone to take pictures and record the color, and use ImageJ analysis software to read the color intensity of the color-developed area;

[0...

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Abstract

The invention belongs to the technical field of paper-based microfluidic analysis, and specifically relates to a multi-element paper-based sliding microfluidic chip based on a cerium metal organic framework as well as a preparation method and a detection method thereof. The multi-element paper-based sliding micro-fluidic chip comprises an upper-layer reaction chip and a lower-layer color developing chip which are matched with each other, wherein a base material of the chips is porous filter paper, both the upper-layer reaction chip and the lower-layer color developing chip comprise a rectangular main body region and a side edge folding wing, and the main body region is divided into a hydrophilic region and a hydrophobic region; and the hydrophilic region comprises a reaction region and a color developing region, the reaction region modifies oxidase corresponding to a detection substance, and the color developing region anchors and assembles Ce-MOF nanoparticles for developing and filtering protein. The paper-based sliding chip is used for developing the color of hydrogen peroxide generated by the corresponding upper-layer reaction chip through the lower-layer color developing chip,a detection result is presented on the chip without expensive instruments and complex data processing processes, so that the multi-element paper-based sliding microfluidic chip is easy to operate, low in cost and wide in application range.

Description

technical field [0001] The invention belongs to the technical field of paper-based microfluidic analysis, in particular to a multi-element paper-based sliding microfluidic chip based on cerium metal organic framework. Background technique [0002] Paper-based microfluidic analysis technology is an emerging development field of microfluidic analysis technology. It has been widely used due to its good portability, low cost, abundant raw material sources, easy recycling, and good biocompatibility. It is suitable for the preparation of disposable portable analysis equipment, and it is easy to operate, and even does not require specially trained professional operators, so it has great application prospects. The statistical results show that, so far, the main detection method of paper-based microfluidic chips is colorimetry, mainly due to the relatively simple reaction conditions, the results are more intuitive and do not require expensive external detection equipment. The detect...

Claims

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Application Information

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IPC IPC(8): G01N21/78B01L3/00
CPCG01N21/78B01L3/502753B01L2300/12B01L2200/10B01L2200/027
Inventor 宋玉君栾小维
Owner NANJING UNIV
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