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Highly elastic water-stable protein-based/epoxy composite microfiber tissue engineering scaffold and preparation method thereof

A tissue engineering scaffold and ultra-fine fiber technology, applied in tissue regeneration, fiber chemical characteristics, cellulose/protein conjugated rayon, etc., can solve the problems of high toxicity and low efficiency, and achieve low cost, good growth, good biocompatibility

Active Publication Date: 2021-09-17
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a preparation technology for a biocompatible protein ultrafine fiber scaffold with high elasticity and high water stability, to overcome the defects of low efficiency and high toxicity of the existing modification technology, and to realize the high efficiency of the scaffold. Elasticity, water stability, high mechanical properties, biocompatibility

Method used

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  • Highly elastic water-stable protein-based/epoxy composite microfiber tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of a protein-based / epoxy composite superfine fiber tissue engineering scaffold:

[0031] Zein and ethylene glycol diglycidyl ether were added into 75% ethanol while stirring at a ratio of 10:3, and the total mass concentration of zein and ethylene glycol diglycidyl ether was 30%. Add polyvinylpyrrolidone to the spinning solution, the proportion is 5% of the zein mass, then stir at 500rpm at room temperature for 30min until it is completely dissolved, and stand at room temperature for 24h to obtain a zein / ethylene glycol diglycidyl ether solution .

[0032]The spinning solution was placed in the solution chamber of a single-needle electrospinning device, the spinning environment was controlled at 25°C, 40% RH, the fiber receiving device was a drum-type receiver, and the rotating speed was 300r / min. The distance from the spinneret is 18cm, the solution injection speed is determined to be 3.0mL / h during the spinning process, and the voltage is maintai...

Embodiment 2

[0035] A preparation method of a protein-based / epoxy composite superfine fiber tissue engineering scaffold:

[0036] Add wheat protein and diethylene glycol diglycidyl ether into 75% acetic acid while stirring at a ratio of 10:3, and the total mass concentration of wheat protein and diethylene glycol diglycidyl ether is 20%. Add sodium dodecylsulfonate and trimethylolpropane into the spinning solution, the proportions are respectively 5% and 1% of the wheat protein mass, then stir at 500rpm at room temperature for 30min until completely dissolved, and stand at room temperature for 24h to obtain wheat protein / diethylene glycol diglycidyl ether solution.

[0037] The spinning solution was placed in the solution chamber of a single-needle electrospinning device, the spinning environment was controlled at 25°C, 50% RH, the fiber receiving device was a drum-type receiver, and the rotating speed was 300r / min. The distance from the spinneret is 16cm, the solution injection speed is ...

Embodiment 3

[0040] A preparation method of a protein-based / epoxy composite superfine fiber tissue engineering scaffold:

[0041] Collagen and 1,4-butanediol diglycidyl ether were added into 75% ethanol while stirring in a ratio of 10:2, and the total mass concentration of collagen and 1,4-butanediol diglycidyl ether was 25%. Add polyvinylpyrrolidone and glycerin to the spinning solution, the proportions are 5% and 1% of the collagen mass, then stir at 500rpm at room temperature for 30min until they are completely dissolved, and stand at room temperature for 32h to obtain collagen / 1,4-butane Alcohol diglycidyl ether solution.

[0042] The spinning solution was placed in the solution chamber of a single-needle electrospinning device, the spinning environment was controlled at 25°C, 50% RH, the fiber receiving device was a drum-type receiver, and the rotating speed was 300r / min. The distance from the spinneret is 16cm, the solution injection speed is determined to be 3.0mL / h during the spi...

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Abstract

The invention discloses a preparation method of a highly elastic water-stable protein-based / epoxy composite superfine fiber tissue engineering scaffold, which is characterized in that a spinning liquid is prepared, wherein the components of the spinning liquid include protein and epoxy compounds and dispersant, and chain extender according to requirements; the spinning solution is electrospun into a scaffold, and then the scaffold is subjected to stabilization enhancement post-treatment and end-capping post-treatment in turn to obtain a highly elastic water-stable protein-based / epoxy composite Microfiber scaffolds for tissue engineering. The scaffold includes micro-nano fibers and a pore structure formed by random stacking of micro-nano fibers, with a porosity of 50-70% and an average pore diameter of 1-5 μm. The invention endows the morphological stability, elasticity and elastic recovery of the protein ultrafine fiber liquid phase, and effectively improves the mechanical properties of the protein ultrafine fiber. The invention broadens the application field of protein-based superfine fiber materials, promotes the development of composite functional tissue repair materials, and can be produced continuously.

Description

technical field [0001] The invention belongs to the field of biomedical textile materials and its preparation, and specifically relates to a tissue engineering biological material and its preparation, in particular to a highly elastic and water-stable protein-based / epoxy composite superfine fiber tissue engineering scaffold and its preparation method. Background technique [0002] Electrospinning technology can quickly and stably prepare ultrafine fibers with sizes ranging from tens of nanometers to several microns, and the selection of raw materials is also wide. The prepared ultrafine fiber system has ultra-high specific specific surface area and porosity, and the structure is similar to the three-dimensional (3D) fiber network in natural extracellular matrices (ECMs), which can promote cell attachment, migration, proliferation, and differentiation It also has efficient drug adsorption and delivery functions. The interconnected pore structure in the structure facilitates ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/24A61L27/56A61L27/58A61L27/50D01F8/02D01F8/16D01F1/10
CPCA61L27/227A61L27/24A61L27/50A61L27/56A61L27/58A61L2430/06A61L2430/10A61L2430/24D01F1/10D01F8/02D01F8/16
Inventor 蒋秋冉刘璐李冉刘飞江建财杨圆
Owner DONGHUA UNIV
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