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A kind of MPC-modified dendrimer-wrapped gold nanoparticles and its preparation and application

An amine dendrimer, 25-G5.NH2-MPC20} technology, which is applied in the field of dendrimer-encapsulated gold nanoparticles, can solve the problems of poor gene-carrying capacity and low biocompatibility, and achieves easy operation, The effect of good biocompatibility and efficient gene loading effect

Active Publication Date: 2022-05-06
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] CN 106512028 A discloses a zwitterion-modified dendrimer-wrapped gold nanoparticle CT contrast agent and its preparation and application. It is only used in tumor imaging, and the ratio of gold particles to dendrimer is 80:1. Poor ability to load genes
10:1 zwitterion to dendrimer ratio, low biocompatibility

Method used

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  • A kind of MPC-modified dendrimer-wrapped gold nanoparticles and its preparation and application
  • A kind of MPC-modified dendrimer-wrapped gold nanoparticles and its preparation and application
  • A kind of MPC-modified dendrimer-wrapped gold nanoparticles and its preparation and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Weigh 3.51 mg of 2-methacryloyloxyethyl phosphorylcholine (MPC) and dissolve it in ultrapure water, then add 20.13 mg of G5.NH 2 Dissolve in ultrapure water and mix well with the above solution, and react for 72 hours. Add 265.72 μL HAuCl to the product of the above reaction according to the molar ratio of dendrimers to gold of 1:25 4 React for 30 minutes, then add 2.19mg NaBH 4 The reaction was stirred for 3 hours. After dialysis for 3 days, the material {(Au 0 ) 25 -G5.NH 2 -MPC 20}.

Embodiment 2

[0040] Weigh 2mg of material ({(Au 0 ) 25 -G5.NH 2 -MPC 20}) dissolved in 500 μL of D 2 O, and then use the NMR spectrometer to measure the H NMR spectrum of the material. NMR results such as figure 2 as shown, 1 H NMR spectrum was used to characterize the grafted G5.NH 2 The number of carbon chains on the G5.NH 2 The proton peak of the characteristic group is between 2.2-3.4ppm, and the proton absorption peak at 1.9ppm is the absorption peak of the methyl group on the No. 1 carbon atom on MPC, indicating that MPC is successfully connected to G5.NH 2 Above, use the origin software to calculate the integral area of ​​the proton peak, and get the average of each G5.NH 2 There are approximately 20.1 MPCs connected to the surface. The results showed that {(Au 0 ) 25 -G5.NH 2 -MPC 20}.

Embodiment 3

[0042] Put the material ({(Au 0 ) 25 -G5.NH 2 -MPC 20}) was dissolved in ultrapure water, prepared into a solution with a concentration of 2 mg / mL, and the ultraviolet absorption value in the range of 300nm-800nm ​​was detected by a ultraviolet-visible spectrophotometer. The results of UV-Vis spectrophotometry were as follows: image 3 As shown, the material wrapping the gold particles has an absorption peak around 520nm, which indicates that the gold nanoparticles are successfully wrapped.

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Abstract

The present invention relates to a kind of MPC-modified dendrimer wrapped nano-gold particles and its preparation and application, comprising dissolving MPC in a solvent, adding polyamide-amine dendrimers dissolved in the solvent to react, and then adding chlorine Auric acid solution and sodium borohydride solution, after dialysis, freeze-drying, that is. The preparation process of the present invention is short and easy to operate; and the material has low toxicity at the dosage, good biocompatibility, can compress the CpG gene, and the compound loaded with the CpG gene can effectively stimulate immune cells and then kill tumor cells, which is very important. The application of viral vectors in tumor immunotherapy provides a reference.

Description

technical field [0001] The invention belongs to the field of tumor immunotherapy, and in particular relates to an MPC-modified dendrimer-wrapped gold nanoparticle and its preparation and application. Background technique [0002] Malignant tumor is a major disease that endangers human health, and its incidence is increasing year by year. With the development of science and the improvement of medical technology, the survival rate of patients with malignant tumors has increased, but the mortality rate has not changed, which is mainly due to the recurrence and metastasis of tumors. At present, the main methods for the treatment of malignant tumors include surgery, radiotherapy, chemotherapy, and combination therapy, but there are many problems such as tumor metastasis, recurrence, and adverse reactions of radiotherapy and chemotherapy. With the development of tumor immunotherapy and modern biotechnology, tumor immunotherapy has become the fourth tumor treatment method after su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/34A61K47/02A61K31/711A61K49/00A61P35/00
CPCA61K9/5146A61K9/5115A61K31/711A61K49/0017A61P35/00
Inventor 曹雪雁陈环史向阳
Owner DONGHUA UNIV
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