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Detection method of crust leather comprehensive toxicity

A detection method and technology of crust leather, applied in the field of comprehensive toxicity detection of crust leather based on luminescent bacteria, can solve the problems of long time, high detection cost, complicated comprehensive toxicity screening operation, etc., achieve short reaction time, good repeatability, The effect of improving detection efficiency and accuracy

Pending Publication Date: 2019-12-24
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the prior art that only detects a single pollutant, but the comprehensive toxicity screening operation is complicated, takes a long time, and the detection cost is high, and provides an easy-to-operate, economical and practical screening method based on luminescent bacteria. Crude leather toxicity test method

Method used

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  • Detection method of crust leather comprehensive toxicity
  • Detection method of crust leather comprehensive toxicity
  • Detection method of crust leather comprehensive toxicity

Examples

Experimental program
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Effect test

Embodiment 1

[0049] A comprehensive toxicity detection method for chrome-tanned leather based on luminescent bacteria, referring to figure 1 , the method includes the steps of:

[0050] (1) Crude leather sample pretreatment: Crude leather samples are from Zhejiang Haining Rising Leather Co., Ltd., sampled in strict accordance with QB / T 2708-2005, dried at 45°C for 48 hours, placed in standard air at constant temperature and humidity for 48 hours, Retsch Cut it into 4×4mm with SM 100 cutter and grinder, seal the sample bag, and store it in standard air with constant temperature and humidity for later use.

[0051] (2) Sample extraction: Weigh 5.0 g of the sample and place it in a 250 mL Erlenmeyer flask, add 100 mL of 0.1% wt NaCl solution adjusted to pH 4.0 with 0.1 M HCl, place it in a ZWY-2102C constant temperature oscillator, and keep the temperature at 25°C , rotate at 100 rpm, shake horizontally for 8 hours, and filter with a 0.45um filter membrane. The filtrate was diluted with 2.5...

Embodiment 2

[0057] A comprehensive toxicity detection method for chrome-tanned leather based on luminescent bacteria, referring to figure 1 , the method includes the steps of:

[0058] (1) Crude leather sample pretreatment: chrome tanned leather scraps were sourced from Zhejiang Star Leather Plastic Co., Ltd. The scraps were sampled in quarters, dried at 45°C for 48 hours, and placed in standard air at constant temperature and humidity for 48 hours , Retsch SM 100 cutting and milling machine chopped to 4 × 4mm, the sample bag was sealed, and stored in standard air at constant temperature and humidity for later use.

[0059] (2) Sample extraction: Weigh 4.0 g of the sample and place it in a 250 mL Erlenmeyer flask, add 120 mL of 0.3% wtNaCl solution with a pH of 7.0, and place it in a ZWY-2102C constant temperature oscillator with a constant temperature of 25°C and a speed of 60 rpm. After shaking for 24 hours, filter with a 0.45um filter membrane, and dilute the filtrate with 2.5% wt NaC...

Embodiment 3

[0068]A comprehensive toxicity detection method for polysaccharide aldehyde tanned leather based on luminescent bacteria, referring to figure 1 , the method includes the steps of:

[0069] (1) Crude leather sample pretreatment; Crude leather was provided by Guangdong Demei Fine Chemical Co., Ltd., sampled in strict accordance with QB / T2708-2005, dried at 45°C for 48 hours, then placed in standard air at constant temperature and humidity for 48 hours, Retsch Cut it into 4×4mm with SM 100 cutter and grinder, seal the sample bag, and store it in standard air with constant temperature and humidity for later use.

[0070] (2) Sample extraction: Weigh 3.0 g of the sample and place it in a 250 mL Erlenmeyer flask, add 120 mL of 0.1% wt NaCl solution adjusted to pH 9.0 with 0.1 M NaOH, place it in a ZWY-2102C constant temperature oscillator, and keep the temperature at 30°C , rotate at 80 rpm, shake horizontally for 24 hours, filter with a 0.45 um filter, add NaCl to the filtrate to ...

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Abstract

The invention discloses a method for detecting crust leather comprehensive toxicity by luminescent bacteria. The method comprises the steps that a NaCl solution with the concentration being lower than2.5% wt is used as an extracting agent to extract weakly bound chemicals from crust leather, and a crust leather extraction solution is obtained; NaCl is added into the extraction solution to make the concentration of the extraction solution be 2.5% wt, and pH is adjusted to be 7.0; or the NaCl solution of 2.5% wt is used for diluting the extraction solution to a suitable concentration gradient,and pH is adjusted to be 7.0; a toxicity test sample is obtained; the cultured luminescent bacteria are taken, a sample solution is added, still standing is conducted after even mixing, and the luminous intensity is measured; and expression of sample toxicity is conducted, specifically, a NaCl solution of 2.5% wt is taken as a blank, and the relative luminous intensity and the half relative luminous intensity concentration EC50 of a mixture after still standing are measured. According to the method, the NaCl solution is used as the extracting agent to extract toxic substances in the crust leather sample, the comprehensive toxicity of the extraction solution is directly detected by a toxicity detector, a convenient method for detection of comprehensive toxicity of the crust leather is constructed, and the shortcomings of a traditional method are avoided.

Description

technical field [0001] The invention relates to the field of comprehensive toxicity detection methods for luminescent bacteria, in particular to a comprehensive toxicity detection method for crust leather based on luminescent bacteria. Background technique [0002] Raw hides are prepared into finished leather after pretreatment, tanning, dyeing and finishing, and finishing, while tanned and unfinished leather is called crust leather. Crust leather contains a variety of chemical substances, such as chromium, aldehydes, phenols, surfactants, resins, etc. These substances may overflow during processing, use and disposal, and become pollutants that endanger health and environmental ecology. These pollutants not only have their own toxicity, but also the mixing and interaction of various pollutants will also produce potential toxicity. Literature "Investigation into the hazards of finished Cr-tanned leather as both product and waste" (Peng LQ , Li Y, Han WM, Long WJ , Zhang WH,....

Claims

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Application Information

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IPC IPC(8): C12Q1/02C12N1/20
CPCC12Q1/025C12N1/20Y02A50/30
Inventor 张文华彭良琼石碧曾运航周建飞王亚楠
Owner SICHUAN UNIV
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