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Target gene identifying method for tumor treatment

A technology of tumor treatment and identification method, which is applied in the field of target gene identification for tumor treatment, can solve the problem that there is no other method that can test whether the gene variation analysis is correct, and achieve the effect of a reliable target gene identification method

Inactive Publication Date: 2019-12-20
SAMSUNG LIFE PUBLIC WELFARE FOUND
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Problems solved by technology

In other words, there is no other method that can test whether the gene variation analysis is correct in the existing methods

Method used

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  • Target gene identifying method for tumor treatment
  • Target gene identifying method for tumor treatment
  • Target gene identifying method for tumor treatment

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Experimental program
Comparison scheme
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Embodiment approach

[0084]

[0085] Obtain and culture glioma (Glioma)

[0086] The research group analyzed somatic variation in 127 tumor samples taken from 52 glioma patients who underwent surgery at Samsung Seoul Hospital (SMC). At this time, the tumors were divided into four groups according to the manner in which the samples were collected (ref. figure 2 ). A sample of approximately 5 x 5 x 5 mm3 for genetic analysis was snap-frozen using liquid nitrogen and then enzymatically isolated a portion of the sample into single cells. Tumor cells were cultured in neurobasal cells containing N2 and B27 supplements (supplement, 0.5×, Invitrogen, respectively), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF, 20 ng / ml each, R&Dsystems). ) medium. Patient-derived cells (PDC) used for culture were not contaminated with mycoplasma.

[0087] Whole Exome Sequencing

[0088] raw data

[0089] Agilent's SureSelect kit was used to capture subgroup DNA (exonic DNA) fragments. I...

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Abstract

A target gene identifying method for tumor treatment according to the present invention comprises the steps of: taking multiple samples from a patient's tumor; analyzing the multiple samples for genetic variation; subjecting the multiple samples to drug screening to measure drug sensitivity of each sample; analyzing tumor heterogeneity on the basis of the genetic variation analysis result and thedrug sensitivity measurement result; and identifying a target gene of the tumor on the basis of the tumor heterogeneity analysis result.

Description

technical field [0001] The present invention relates to a target gene identification method for tumor treatment, in particular, to a target gene identification method for collecting multiple tumor samples to find out the ancestral mutation of the tumor through gene variation analysis and drug screening method. Background technique [0002] Tumors are clumps of cells that grow abnormally due to genetic changes in the cells. At this time, various secondary genetic alterations occur from ancestral genetic alterations that lead to initial tumor development, and therefore, tumors can have various genetic mutations depending on their cells. Therefore, it is difficult to decide which gene to choose as the target gene to treat these tumors. [0003] For example, when a first tumor and a second tumor occur in a patient, if the drug used to treat the first tumor targets a gene mutation that occurs only in the first tumor, the drug may have no effect on the second tumor, or even Sec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/00G16B40/00C12Q1/68
CPCG16B20/00G16B40/00C12Q1/6886C12Q2600/106C12Q1/6827C12Q2535/122G16H10/00C12Q1/6869C12Q2600/136C12Q2535/101
Inventor 南都铉李振求J·K·H·史
Owner SAMSUNG LIFE PUBLIC WELFARE FOUND
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