Specific protein marker for ovarian cancer diagnosis and preparation method of protein marker
A technology for protein labeling and ovarian cancer, applied in the field of biomedicine, to achieve strong maneuverability, high sensitivity and specificity, and strong reproducibility
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[0032] One: The present invention is based on the mass spectrometry platform to detect the identification and screening of specific biological protein markers in ovarian cancer serum exosomes:
[0033] 1. Collection of experimental materials:
[0034]Sera were collected from normal healthy people, 9 patients with benign ovarian cystadenoma and 9 patients with malignant ovarian tumors. 5mL whole blood was collected using BD’s vacuum blood collection tube, centrifuged immediately at 3000rpm, transferred the supernatant to a new centrifuge tube, and frozen at minus 80°C.
[0035] 2. Main experimental methods:
[0036] TMT technology is a peptide in vitro labeling technology developed in recent years, which provides convenience for comparing the relative content of proteins in multiple groups of different samples at the same time. The invention conducts quantitative proteome research on samples by organically combining a series of cutting-edge technologies such as TMT labeling, ...
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