Device and method for cell breaking on piezoelectric substrate

A piezoelectric substrate and cell crushing technology, applied in the field of surface acoustic wave cell crushing devices and cell crushing devices, can solve the problems of complex manufacturing process, difficult integration and microfluidic system, and achieve no heating unit, solve Effects of cellular content damage

Inactive Publication Date: 2019-12-20
CHANGZHOU INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main advantage of this method is that it does not require chemical reagents, thereby avoiding the pollution of chemical reagents to the cell contents, but it requires a relatively complicated manufacturing process, and requires an external driving source such as a syringe pump to drive the cell fluid fluid, which is not easy to integrate with the microfluidic system. Room for improvement

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  • Device and method for cell breaking on piezoelectric substrate

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Embodiment Construction

[0017] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0018] The structure of the device for cell disruption on a piezoelectric substrate by surface acoustic waves is as follows: figure 1 As shown, it includes PCB board 1, piezoelectric substrate 2, pins on PCB board (including first pin 3, second pin 4, third pin 5, fourth pin 6), interdigitated The transducer 7 is a reflection grid 8, Teflon AF1600 hydrophobic thin layer 9 and a side wall with a thickness of 1-2 mm.

[0019] The polydimethylsiloxane spacer 10 has a height of 5-6 mm and is attached to the piezoelectric substrate. It is used to support the polydimethylsiloxane crossbar 11 on it, and the embedded width of the crossbar is 1 - 2 mm thin blade 12, the bottom of the thin blade is 0.2-0.3 mm away from the piezoelectric substrate, so as to avoid the thin blade from damaging the piezoelectric substrate and be as close as possible to the...

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Abstract

The invention discloses a device and a method for cell breaking on a piezoelectric substrate. The device comprises a PCB; the PCB is connected with an external circuit through an electrical connectionend; the piezoelectric substrate is attached to the PCB; a hydrophobic thin layer is arranged on the piezoelectric substrate; side walls are arranged on the two sides of the hydrophobic thin layer; an interdigital transducer and a reflecting grating are arranged at the two ends of each side wall; a cross rod is erected above the hydrophobic thin layer; a blade close to the piezoelectric substrateis arranged on the cross rod; and a gap is reserved between the blade and the piezoelectric substrate. Without a high pressure, external driving of an injection pump, a heating unit and any chemicalreagent, the cell breaking on a micro-fluidic device can be realized, so that the problems of cell content damage and external driving source integration incapability in an existing method for cell breaking on the micro-fluidic device are solved.

Description

technical field [0001] The invention relates to a device for breaking cells in a microfluidic chip, in particular to a device and method for realizing cell breaking by surface acoustic waves, and belongs to the technical field of cell breaking. Background technique [0002] Cell disruption is a prerequisite for cell analysis, especially in microfluidic cell analysis, where on-chip cell disruption is required to achieve on-chip cell analysis, and a cell disruption unit must be integrated in the microfluidic analysis system. There are many macroscopic cell disruption methods, and the cell disruption technology is relatively mature. The common method is the mechanical crushing method, which uses high pressure to squeeze the cell fluid sample through a fixed small nozzle at high speed, and hits the impact target to break the sample cells. This method needs to generate high-pressure equipment, and needs to make a high-pressure chamber to accommodate the sample. At the same time,...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/33C12N13/00
CPCC12M47/06C12N13/00
Inventor 董良威
Owner CHANGZHOU INST OF TECH
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