Method for preserving length of rice stigma

Abstract

The invention discloses a method for preserving the length of a rice stigma. According to the method provided by the invention, with clean water as a control, stigmas with 15 different types of materials are treated by utilizing 6 preserving solutions in different component proportions; and in 120 days from the time of sampling, the lengths of the stigmas are measured in 10 periods of time to analyze the influence of the preserving solutions in different component proportions on the preserving effects of the lengths of the stigmas. Results show that S7 (composed of 5 ml of formaldehyde with aconcentration of 38%, 5 ml of glacial acetic acid, 85 ml of alcohol with a concentration of 50% and 5 ml of glycerol) can preserve the length of a stigma not to be changed in 30 days, and has good effects compared with remaining 5 preserving solutions which can only maintain the length of the rice stigma for 2 to 7 days. By utilization of a selected preserving solution S7 to preserve the stigma, the problem of stigma length reduction caused by incapability of measuring in time due to over-large test population and concentrated flowering time can be solved; and an important significance on mining, cloning and functional verification for the gene of the length of the stigma is achieved.

Description

technical field [0001] The invention belongs to the field of investigation methods for rice outcrossing-related traits, and in particular relates to a method for preserving rice stigma lengths. Background technique [0002] Rice is the second largest food crop in the world, and more than 50% of the world's population uses rice as the main source of food. As the largest rice producer in the world, my country has a total annual output of more than 200 million tons, and about 700 million people use rice as a staple food. With the continuous growth of population and the decrease of arable land at the rate of millions of acres per year, it is an inevitable choice to greatly increase the output per unit area to ensure food security. Hybrid rice, which began in my country in the 1970s, has an increase of about 15% per unit area compared with conventional rice at the same maturity period, and its commercial planting has greatly improved rice productivity. Practice has proved that ...

AI Technical Summary

Problems solved by technology

The use of S7 to preserve rice stigmas can solve the problem of stigma length reduction caused by too large experimental populations and concentrated flowering stages that cannot be measured in time, which is of great significance for the mining, cloning and functional verification of stigma length genes

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