A kind of preservation method of rice stigma

Abstract

The invention discloses a method for preserving the stigma length of rice. In this method, 15 stigmas of different types of materials are treated with 6 kinds of preservation solutions with different composition ratios, using clear water as a control. From sampling to 120 days, the length of stigmas is measured in 10 stages, and the preservation of different composition ratios is analyzed. The effect of liquid on the preservation effect of column head length. It was found that S7 (38% formaldehyde 5ml: glacial acetic acid 5ml: 50% alcohol 85ml: glycerol 5ml) can preserve the stigma length for 30 days without any change, compared with other 5 kinds of preservation solutions that can only maintain the rice stigma length for 2‑7 days The effect is good. Preserving stigmas with the screened S7 preservation solution can solve the problem of shrinking stigma lengths caused by too large test populations and concentrated flowering stages that cannot be measured in time, which is of great significance for the mining, cloning and functional verification of stigma length genes.

Description

technical field [0001] The invention belongs to the field of investigation methods for rice outcrossing-related traits, and in particular relates to a method for preserving rice stigma lengths. Background technique [0002] Rice is the second largest food crop in the world, and more than 50% of the world's population uses rice as the main source of food. As the largest rice producer in the world, my country has a total annual output of more than 200 million tons, and about 700 million people use rice as a staple food. With the continuous growth of population and the decrease of arable land at the rate of millions of acres per year, it is an inevitable choice to greatly increase the output per unit area to ensure food security. Hybrid rice, which began in my country in the 1970s, has an increase of about 15% per unit area compared with conventional rice at the same maturity period, and its commercial planting has greatly improved rice productivity. Practice has proved that ...

AI Technical Summary

Problems solved by technology

The use of S7 to preserve rice stigmas can solve the problem of stigma length reduction caused by too large experimental populations and concentrated flowering stages that cannot be measured in time, which is of great significance for the mining, cloning and functional verification of stigma length genes

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