Method for rapidly and accurately measuring content of vitamin C in flue-cured tobacco

Abstract

The invention discloses a method for rapidly and accurately measuring the content of vitamin C in flue-cured tobacco. The method comprises taking a vitamin C standard solution, adding distilled waterto a constant volume to obtain a plurality of I-dimensional C solutions with different concentrations, taking the distilled water as a reference, determining the absorbance I of each concentration-dimensional vitamin C solution at the maximum absorption wavelength, taking the concentration I and the absorbance I of each concentration-dimensional C solution as a standard curve, and obtaining a corresponding formula according to the standard curve; crushing and sieving to-be-detected tobacco leaves, putting a tobacco powder sample into a container, adding hydrochloric acid and activated carbon,oscillating in a shaking table, filtering, sucking filtrate, and adding distilled water to a constant volume for later use; and taking distilled water as a blank, measuring the volume-fixed solution by using an ultraviolet spectrophotometry to obtain the absorbance II of the volume-fixed solution, finding out the corresponding concentration II from the standard curve according to the absorbance II, and calculating the content of vitamin C in the tobacco to be measured according to the concentration II. According to the method, the content of vitamin C in the flue-cured tobacco can be rapidly and accurately measured, and compared with other methods, the method is cheap and high in reproducibility.

Description

technical field [0001] The invention belongs to the technical field of tobacco detection, and in particular relates to a method for quickly and accurately measuring the content of vitamin C in flue-cured tobacco, which is simple, feasible, accurate, reliable and has good reproducibility. Background technique [0002] Vitamin C, also known as ascorbic acid, is widely found in fruits, foods, Chinese medicinal materials, and plants. It is a water-soluble vitamin that can prevent gum bleeding and shrinkage, improve human immunity, and have certain effects on scurvy, arteriosclerosis, and anemia. curative effect. In the existing technology, the most conventional determination methods of vitamin C content include ultraviolet light spectrophotometry, iodometric method, potentiometric titration, 2,6-dichlorophenol indophenol titration, high performance liquid chromatography, fluorescence photometry method, atomic absorption method, etc., but the instruments of atomic absorption met...

AI Technical Summary

Problems solved by technology

But in the prior art, adopt ultraviolet light spectrophotometry to detect the vitamin C content in the tobacco leaf, can cover vitamin C content because of the chlorophyll in the tobacco leaf and phenolic substance, cause detection data inaccurate; And when the sample processing before detection, The solvent used may also cause the instability of vitamin C, thereby affecting the reproducibility of the test results; in addition, the inappropriate UV wavelength and solvent coordination during the detection of ultraviolet spectrophotometry will also make other substances contained in the tobacco leaves and solvents interfere with the detection, thus affecting the accuracy and reproducibility of the detection results

Due to the aforementioned reasons, the ultraviolet spectrophotometric method for the detection of vitamin C content in tobacco leaves is not currently used as one of the routine options for tobacco leaf detection, and is only discussed and applied in some experiments.

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