White blood cell extraction device

Abstract

The invention discloses a white blood cell extraction device. The white blood cell extraction device successively includes five parts of an upper cover plate, an extraction module, a storage module, alysis module and a lower cover plate from top to bottom, the upper cover plate is provided with a white blood cell channel, a lysed red blood cell channel and a normal saline channel, the white bloodcell channel and the lysed red blood cell channel are respectively provided with an outlet, and the normal saline channel is provided with an inlet; and the extraction module is provided with a helical extraction channel, a white blood cell channel and a lysed red blood cell channel, the storage module is provided with a reaction channel, the lysis module is provided with a mixing channel, and the lower cover plate is provided with a blood channel and a red blood cell lysate channel. According to the white blood cell extraction device, high-activity white blood cells can be automatically, rapidly and steadily extracted from undiluted blood, lysed red blood cells are removed at the same time, and a quick and effective way is provided for application such as clinical diagnosis of subsequentdiseases.

Description

technical field [0001] The invention relates to a cell separation device, in particular to a white blood cell extraction device. Background technique [0002] Leukocytes in human blood generally account for 0.1% of the total blood cells in the human body, and the rich information they contain can be used for the diagnosis of many diseases. For example: coronary heart disease, cancer, etc. The current mainstream leukocyte extraction method is to first lyse red blood cells with red blood cell lysate, and then centrifuge multiple times to remove the lysed red blood cells to leave white blood cells. However, this method is time-consuming and labor-intensive, and requires manual operation by operators with certain experience, and the centrifugal force during centrifugation has been proved to be harmful to the activity of cells. [0003] In recent years, active microfluidic devices have been greatly developed. They usually sort leukocytes according to the different dielectric pr...

AI Technical Summary

Problems solved by technology

However, this method is time-consuming and labor-intensive, requiring manual operation by operators with certain experience, and the centrifugal force during centrifugation has been proved to be harmful to the activity of cells

[0003] In recent years, active microfluidic devices have been greatly developed. They usually sort leukocytes according to the different dielectric properties and magnetic susceptibility of different cells. However, the blood processing speed of these methods is extremely low, usually only one minute per minute. Process 1-10 µl of blood

The leukocyte extraction device based on inertial microfluidics usually needs to dilute the blood 50-100 times in advance to extract leukocytes, so the pre-dilution operation increases the cumbersome operation and reduces the blood processing speed

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