FPM-based micro imaging system

Abstract

The invention discloses an FPM-based micro imaging system. The system comprises a digital micro mirror, a micro lens array, a laser used for emitting light to the digital micro mirror for enabling thedigital micro mirror to reflect the light to the lens array, and an illumination objective lens used for converting spherical waves into plane waves after the micro lens array converts the plane waves into the spherical waves and transmits the light to the illumination objective lens, and irradiating the light to an imaging object placed on an object carrying hole to perform imaging. According tothe system, a to-be-tested sample can be illuminated at different angles by cooperation of the digital micro mirror and the micro lens array; an imaging instrument obtains a low-resolution image; andthen an FPM algorithm is used for iterative reconstruction, so that the image resolution is improved and is equivalent to the resolution of the high-power objective lens, the wider field of view is achieved, the energy of the system is improved, the signal-to-noise ratio is reduced, and the imaging speed is improved.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to an FPM-based microscopic imaging system. Background technique [0002] In related technologies, due to the limitation of the space-bandwidth product of the optical microscope itself, its information flux is certain. To obtain high-resolution images, the numerical aperture must be increased, but the field of view is limited. FPM (FourierPtychography Microscopy, frequency-domain stacked microscope) can bypass the resolution limit of the objective lens, greatly improve the spatial bandwidth product of the system, and enable the objective lens with a small numerical aperture to obtain higher frequency information, which can only obtain low-frequency information. Directional light to improve resolution. [0003] However, the existing FPM-based microscopic illumination systems generally use LEDs as light sources, which have the characteristics of small field of view, low ...

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Problems solved by technology

[0003] However, the existing FPM-based microscopic illumination system generally uses LED as the light source, which has the characteristics of small field of view, low energy, and low signal-to-noise ratio.

There is still a contradiction between the number of openings of digital micromirrors and the signal-to-noise ratio and resolution in the microscopic illumination system using laser light source

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