Capillary chromatographic column device and liquid chromatographic separation method

Abstract

The invention discloses a capillary chromatographic column device and a liquid chromatographic separation method. The capillary chromatographic column comprises a first capillary tube and a second capillary tube, wherein the first capillary tube is used for being filled with a separation column material, the outlet end of the first capillary tube is of a pointed conical structure, and an outlet islocated in the end of the pointed conical structure; and the inlet end of the second capillary tube is detachably connected with the outlet end of the first capillary tube. Since the outlet end of the first capillary tube is arranged to be the pointed conical structure, the pointed conical structure can block the separation column material, it is needless to prepare a chemical reagent or producea mesh-shaped screening plate, the structure is simpler, manufacturing is easier, and cost is low; moreover, the pointed conical structure has a physical blocking function, so that corrosive consumption of the chemical screening plate is avoided, and the durability is improved; through the device, low-speed separation of micro-samples is realized, large-volume non-specific absorption loss of the samples is avoided, and the quantity of residues of the samples in the separation column material is small; and the separation column material does not need cleaning or just needs simple and rapid cleaning, and therefore cross contamination among the samples is effectively reduced.

Description

technical field [0001] The invention relates to the technical field of chromatographic separation, in particular to a capillary chromatographic column device and a liquid chromatographic separation method. Background technique [0002] In the rapidly developing field of life sciences, proteomics plays an important role, especially high-throughput proteomics research based on liquid chromatography-mass spectrometry (LC-MS). In addition to the development of the mass spectrometer's own technical performance, the development of liquid chromatography separation (grouping) technology has also played a key role in improving proteomics or metabolomics research. Liquid chromatographic separation is further divided into primary separation (pre-grouping) and secondary separation (nanoliter separation by mass spectrometry), in which the effect of primary separation is the decisive factor for the depth and coverage of proteome identification. [0003] There are three main technical sch...

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Problems solved by technology

[0004] In the existing primary separation technology method, the chromatographic column specification and flow rate used for HPLC separation column separation are relatively large (the flow rate is usually 200ul / min-2000ul / min). Residues lead to cross-contamination; on the other hand, the volume obtained after separation is relatively large, and the sample concentration is low, which is likely to cause secondary losses during subsequent drying or detection, so its separation sensitivity and application range of sample volume are relatively limited. At the same time, commodity The cost of the separation column is also higher

Both spin column centrifugal separation and stage-tip hand-push separation have problems such as cumbersome operation, poor reproducibility, low separation resolution, low separation stages, high requirements for sample initial volume, and large elution volume. Its separation simplicity, reproducibility, sensitivity, separation efficiency and scope of application are relatively limited

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