Full-automatic nucleic acid extraction and amplification micro-fluidic chip dynamic quantitative detection integrated device

Abstract

The invention relates to a full-automatic nucleic acid extraction and amplification micro-fluidic chip dynamic quantitative detection integrated device which comprises a nucleic acid automatic extraction module for automatically cracking and purifying nucleic acids in one or more samples, a nucleic acid amplification and hybridization module, and a dynamic quantitative detection module; the nucleic acid amplification and hybridization module comprises a micro-fluidic chip, and the micro-fluidic chip can receive the purified nucleic acids from the nucleic acid automatic extraction module and carry out PCR amplification and hybridization; the dynamic quantitative detection module is used for scanning and imaging the micro-fluidic chip through a CCD camera so as to detect the micro-fluidic chip. A mechanical arm can be used for completing the continuous sample loading function of a plurality of sample containers, automatic detection of large samples is realized, scanning imaging can be carried out by the CCD camera when each time of PCR amplification is completed, dynamic quantitative detection is realized, and quantitative analysis is carried out on the samples by synthesizing the whole amplification and hybridization process, so that the accuracy of a digital PCR technology is improved.

Description

technical field [0001] The invention relates to the field of biological detection and molecular diagnosis, in particular to an integrated device for automatic nucleic acid extraction and amplification microfluidic chip dynamic quantitative detection. Background technique [0002] In 1953, Watson and Crick proposed the double helix structure model of deoxyribonucleic acid, which was a milestone achievement and laid a solid theoretical foundation for the development of molecular biology and the advancement of molecular diagnostic technology. The development of molecular diagnosis has gone through four stages, including molecular hybridization technology, PCR technology, biochip technology, and second-generation sequencing technology. In recent years, molecular diagnosis is rapidly becoming one of the most effective ways to diagnose human diseases. Among them, biochip technology solves the problems of traditional nucleic acid imprinting and hybridization technology, which are c...

AI Technical Summary

Problems solved by technology

However, at present, molecular diagnostic products in the world, including Bio-Rad, ABI, BD, and Roche, are basically based on real-time fluorescent quantitative PCR technology and digital PCR technology. The most important nucleic acid sample processing, PCR, and nucleic acid detection machines are all completed by several separate machines. , the operation of the sub-system is cumbersome, the degree of intelligence is not high, the cost is high, pollution is easy to occur, and the operation of several instruments is easy to cause human error

At present, only Xpert RIF product is highly integrated with nucleic acid sample extraction, PCR and detection in one machine, but it is very expensive

my country does not have relevant mature technologies and products

In addition, the disadvantage of this product is that it can only detect the final PCR result and cannot perform real-time fluorescence quantitative analysis

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