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A kind of hypothetical protein gp075 of pseudomonas aeruginosa bacteriophage k8 and its mutant strain, mutant protein and application

A Pseudomonas aeruginosa and hypothetical protein technology, applied in the field of bioengineering, can solve problems such as patent publications that have not yet been found, achieve strong lysing and adsorption of host cells, solve infection, and wide host range effects

Active Publication Date: 2022-04-08
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • A kind of hypothetical protein gp075 of pseudomonas aeruginosa bacteriophage k8 and its mutant strain, mutant protein and application
  • A kind of hypothetical protein gp075 of pseudomonas aeruginosa bacteriophage k8 and its mutant strain, mutant protein and application
  • A kind of hypothetical protein gp075 of pseudomonas aeruginosa bacteriophage k8 and its mutant strain, mutant protein and application

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Embodiment Construction

[0031] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0032] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0033] A hypothetical protein GP075 of Pseudomonas aeruginosa phage K8, wild-type GP075 protein, its nucleotide sequence is SEQ No.1, and its amino acid sequence is SEQ No.10.

[0034] A Pseudomonas aeruginosa phage K8 mutant strain K8-D7, said mutant strain K8-D7 can infect lipopolysaccharide O-antigen-deficient host cells, its sequence is amino acid 105, 106 in the hypothetical protein GP075 as described above An amino acid sequence that completely repeats 106 to 112 is inserted between them; the nucleotide sequence...

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Abstract

The present invention relates to a hypothetical protein GP075 of Pseudomonas aeruginosa bacteriophage K8, whose nucleotide sequence is SEQ No.1, and whose amino acid sequence is SEQ No.10. The hypothetical protein GP075 is mutated, and the mutated protein has the function of a structural protein. On the basis of the original recognition of lipopolysaccharide, this type of phage mutant strain has added an additional receptor recognition protein, which can recognize lipopolysaccharide O-antigen-deficient host cells Or host cells that only contain core oligosaccharides, and have a wider host range, stronger ability to lyse and adsorb host cells, and are expected to be used in phage preparations to prevent and treat various diseases caused by Pseudomonas aeruginosa. Infect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a hypothetical protein GP075 of Pseudomonas aeruginosa phage K8 and its mutant strain, mutant protein and application. Background technique [0002] Pseudomonas aeruginosa is an opportunistic pathogen that easily infects immunocompromised, debilitated and cystic fibrosis patients, resulting in higher morbidity and mortality in cystic fibrosis patients. As the natural killer of bacteria, phage can specifically kill bacteria, and the study of the interaction between phage and host will help to develop phage preparations for the treatment of bacterial infections. In recent years, the research on Pseudomonas aeruginosa and its phages has been increasing day by day, and the infection mechanism of various phages of Pseudomonas aeruginosa has become more and more clear. Most of the isolated Pseudomonas aeruginosa phage recognition receptors are lipopolysaccharide O- Antigens, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/005C12N7/00C12R1/92
CPCC07K14/005C12N7/00C12N2795/00022C12N2795/00011
Inventor 杨洪江孙利张志强李东航尤甲甲
Owner TIANJIN UNIV OF SCI & TECH
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