Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pseudomonas aeruginosa phage K8 hypothetical protein GP075, mutant strain, mutant protein and application thereof

A Pseudomonas aeruginosa and hypothetical protein technology, applied in the field of bioengineering, can solve problems such as patent publications that have not yet been found, achieve strong lysing and adsorption of host cells, improve bactericidal ability, and wide host range

Active Publication Date: 2019-12-13
TIANJIN UNIV OF SCI & TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pseudomonas aeruginosa phage K8 hypothetical protein GP075, mutant strain, mutant protein and application thereof
  • Pseudomonas aeruginosa phage K8 hypothetical protein GP075, mutant strain, mutant protein and application thereof
  • Pseudomonas aeruginosa phage K8 hypothetical protein GP075, mutant strain, mutant protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0032] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0033] A hypothetical protein GP075 of Pseudomonas aeruginosa phage K8, wild-type GP075 protein, its nucleotide sequence is SEQ No.1, and its amino acid sequence is SEQ No.10.

[0034] A Pseudomonas aeruginosa phage K8 mutant strain K8-D7, said mutant strain K8-D7 can infect lipopolysaccharide O-antigen-deficient host cells, its sequence is amino acid 105, 106 in the hypothetical protein GP075 as described above An amino acid sequence that completely repeats 106 to 112 is inserted between them; the nucleotide sequence...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to pseudomonas aeruginosa phage K8 hypothetical protein GP075, its nucleotide sequence is SEQ No.1 and its amino acid sequence is SEQ No.10. The hypothetical protein GP075 is subjected to mutation; the mutated protein has the function of structural protein; the phage mutant strain is based on the original recognition of lipopolysaccharide, extra receptor recognition protein is added, the lipopolysaccharide O-antigen-deficient host cell or the host cell only containing the core oligosaccharides can be identified, such that the hypothetical protein has a wider host range and stronger capability of cracking and adsorbing host cells, and is expected to be applied to bacteriophage preparations for preventing and treating various infections caused by pseudomonas aeruginosa.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a hypothetical protein GP075 of Pseudomonas aeruginosa phage K8 and its mutant strain, mutant protein and application. Background technique [0002] Pseudomonas aeruginosa is an opportunistic pathogen that easily infects immunocompromised, debilitated and cystic fibrosis patients, resulting in higher morbidity and mortality in cystic fibrosis patients. As the natural killer of bacteria, phage can specifically kill bacteria, and the study of the interaction between phage and host will help to develop phage preparations for the treatment of bacterial infections. In recent years, the research on Pseudomonas aeruginosa and its phages has been increasing day by day, and the infection mechanism of various phages of Pseudomonas aeruginosa has become more and more clear. Most of the isolated Pseudomonas aeruginosa phage recognition receptors are lipopolysaccharide O- Antigens, th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/005C12N7/00C12R1/92
CPCC07K14/005C12N7/00C12N2795/00022C12N2795/00011
Inventor 杨洪江孙利张志强李东航尤甲甲
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com