Application of outer membrane protein in preparation of malignant tumor immunotherapy drugs
A technology for immunotherapy drugs and outer membrane proteins, applied in the field of medicine, can solve problems such as undisclosed effects, and achieve the effect of inhibiting tumor growth and reducing tumor load
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[0044] 1. Outer membrane protein expression
[0045] 1. Use Escherichia coli engineering bacteria BL21 strain for heterologous expression, high-efficiency expression of genes cloned in expression vectors (such as pET series) containing phage T7 promoter, the plasmid PET 28a-Amuc used in this experiment, the map is as attached figure 1 , the Amuc protein gene is introduced into PET 28a through genetic engineering, and the plasmid PET28a-Amuc containing the Amuc protein gene is constructed. The method of constructing the plasmid is a conventional genetic engineering technique, which will not be repeated here. Cultivate overnight on LB+KanR resistant plate, select monoclonal antibody, transfer to 100ml LB+KanR resistant liquid medium, overnight, transfer to 200ml LB+KanR resistant liquid medium according to the ratio of 1:100, 37 Cultivate at ℃ for 2-3 hours, so that OD=0.6, 8000r / min, centrifuge for 5min, discard the supernatant, and st...
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