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A kind of anti-breast cancer nano drug and preparation method thereof

An anti-breast cancer, nanotechnology, applied in the field of biomedicine, to achieve good monodispersity and stability, rapid release, and relieve side effects.

Active Publication Date: 2020-09-01
苏鑫
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there is currently no relevant report on making saikosaponin D (Ssd) as an active ingredient and using PLGA as a carrier to make targeted nanomedicine

Method used

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  • A kind of anti-breast cancer nano drug and preparation method thereof
  • A kind of anti-breast cancer nano drug and preparation method thereof
  • A kind of anti-breast cancer nano drug and preparation method thereof

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Embodiment

[0042] 1. Cell Culture

[0043] MCF-7, 4T-1, RAW264.7 cell lines were purchased from American Type Culture Collection. The LO2 cell line was obtained from the Institute of Basic Medicine, Chinese Academy of Medical Sciences. All cells were grown in DMEM with 10% fetal bovine serum (FBS) (LifeTechnology) at 37°C, 5% CO 2 Cultured in a constant temperature incubator.

[0044] 2. Extraction of RAW264.7 cell membrane

[0045] Macrophage membranes were isolated from RAW264.7 cells. Take 1×10 6 Cells were washed 3 times with 1×PBS. Cells were suspended in low temperature hypotonic lysis buffer (20 mM Tris-HCl, pH 7.4; 0.10 mM MgCl 2 ; 10 mM KCl; trypsin lysate) and extruded 20 times with a polycarbonate membrane micro-extruder. The resulting cell homogenate was centrifuged at 3200 g for 5 min at 4°C. The supernatant was collected and centrifuged at 20,000 g for 30 min, and the supernatant was left, and then centrifuged at 80,000 g for 1.5 h with an ultracentrifuge (LE-80K, B...

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Abstract

The invention relates to an anti-breast cancer targeting T7-cell membrane-PLGA-saikoside D (Ssd) bionic nano-drug SCMNPs and a preparation method thereof. The method mainly comprises the following steps: 1) extracting RAW264.7 original cell membrane vesicles; 2) synthesizing an Ssd-PLGA drug-loaded nano core; (3) synthesizing T7 peptide coupled pegylated phospholipid (T7-PEG-DSPE); and (4) carrying out fusion loading on the cell membrane-T7-PEG-DSPE vesicles and the drug-loaded Ssd-PLGA nano particles, so as to obtain the nano-drug. The nano-drug has a typical core-shell structure and good monodispersity and stability. The Ssd drug loading capacity is high, the process of releasing the SsD from the SCMNPs is time-dependent, the SsD is rapidly released in a tumor environment, and relievingof side effects during chemotherapy is facilitated. The SCMNPs nano-drug has the capability of targeting tumors, and can selectively inhibit the growth of breast cancer cells at a relatively low drugconcentration.

Description

[0001] Field of Invention [0002] The invention belongs to the technical field of biomedicine, and in particular relates to a nano-drug complex of saikosaponin D-loaded poly(D,L-lactic-co-glycolic acid) for treating breast cancer and a preparation method thereof. [0003] technical background [0004] Breast cancer is the second most common cancer in the world and the most common cancer in women. In 2012, the National Cancer Center and the Department of Disease Control and Prevention of the Ministry of Health released a set of data on female cancer in my country. The data shows that the incidence of breast cancer in my country ranks first among female malignant tumors. The statistics of the incidence of female breast cancer (crude rate) are as follows: 42.55 / 100,000 nationwide, 51.91 / 100,000 in cities, and 23.12 / 10 in rural areas million people. At present, in clinical treatment, the conventional treatment for breast cancer is chemotherapy, but chemotherapy will bring many si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/34A61K47/42A61K47/46A61K31/7048A61P35/00
CPCA61K9/5031A61K9/5052A61K9/5068A61K31/7048A61P35/00
Inventor 苏鑫
Owner 苏鑫
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