Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of pH responsive mesoporous silica nano-drug carrier for three-in-one therapy

A technology of mesoporous silica and nano-drug carrier, which is applied in preparations for in vivo experiments, drug combinations, drug delivery, etc., can solve difficult problems, achieve stable modification process, improve tumor cytotoxicity, and improve imaging The effect of precision

Inactive Publication Date: 2019-12-06
SHANXI UNIV
View PDF2 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problem that it is difficult to realize combined therapy of chemotherapy, chemodynamic therapy and photodynamic therapy by simple means on the same nano-platform, and provides a three-in-one pH therapy for photodynamic therapy / chemodynamic therapy / chemotherapy Preparation method of responsive mesoporous silica nano drug carrier

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of pH responsive mesoporous silica nano-drug carrier for three-in-one therapy
  • Preparation method of pH responsive mesoporous silica nano-drug carrier for three-in-one therapy
  • Preparation method of pH responsive mesoporous silica nano-drug carrier for three-in-one therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037](1) Add 240 mL of water, 0.5 g of surfactant CTAB, and 1.7 mL of 2M sodium hydroxide into a 500 mL volumetric flask, stir slowly and heat up to 80°C, then increase the speed and stir vigorously for 30 minutes, then add 2.5 mL dropwise TEOS, a white suspension was obtained after two hours of reaction. centrifuged, washed twice with ethanol, and redispersed in a mixed system of 140 mL of absolute ethanol and 6 mL of concentrated hydrochloric acid, refluxed for 24 hours, washed by centrifugation, and dried in vacuum to obtain a fine white powder, and obtained mesoporous silica nanoparticles ( MSN).

[0038] (2) 200 mg MSN was ultrasonically dispersed in 100 mL Tris-HCl, 100 mg dopamine was added, and polydopamine-modified silica nanoparticles MP were obtained after stirring for 12 hours in the dark. During the reaction, the color of the system changes from milky white-light pink-gray-grey black-black.

[0039] (3) Weigh 200 mg of MP solids and ultrasonically disperse them...

Embodiment 2

[0043] (1) Add 240 mL of water, 0.5 g of surfactant CTAB, and 1.7 mL of 2M sodium hydroxide into a 500 mL volumetric flask, stir slowly and heat up to 80°C, then increase the speed and stir vigorously for 30 minutes, then add 2.5 mL dropwise TEOS, a white suspension was obtained after two hours of reaction. Centrifuged, washed twice with ethanol, redispersed in 140 mL of 10 mg / mL ammonium nitrate-ethanol mixture, refluxed for 24 hours, washed by centrifugation, dried in vacuum to obtain a fine white powder, and obtained mesoporous silica nanoparticles (MSN ).

[0044] (2) 200 mg MSN was ultrasonically dispersed in 100 mL Tris-HCl, 200 mg dopamine was added, and polydopamine-modified silica nanoparticles MP were obtained after stirring for 12 hours in the dark.

[0045] (3) Weigh 200 mg of MP solids and ultrasonically disperse them in 20 mL of absolute ethanol; dissolve manganese acetate in 30 mL of absolute ethanol to make a 20 mM manganese acetate solution, mix them and stir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of a pH responsive mesoporous silica nano-drug carrier for three-in-one therapy, belongs to the field of drug carrier preparation, and can solve the problem that the difficulty for realizing combined therapy on the same nano-platform through easy means is high currently. The carrier is composed of mesoporous silica nano-particles loaded with an anti-cancer drug, a polydopamine shell layer complex with divalent manganese ions and a photosensitizer; after the nano-carrier is ingested by tumour cells, the polydopamine shell layer is degraded in a subacid environment; the pH responsive anti-cancer drug and the photosensitizer are triggered to be released; methylene blue reacts with dissolved oxygen in the cells under irradiation of 635nm laser, sothat a hydroxyl radical having high toxicity is generated; simultaneously, the divalent manganese ions complex on the polydopamine performs Fenton-like reaction with hydrogen peroxide in the cells, sothat generation of the hydroxyl radical having high toxicity is further promoted; and thus, the three-in-one therapeutic process of photodynamic therapy / chemodynamic therapy / chemotherapy is realized.

Description

technical field [0001] The invention belongs to the technical field of drug carrier preparation, and in particular relates to a method for preparing a pH-responsive mesoporous silica nanometer drug carrier for three-in-one photodynamic therapy / chemodynamic therapy / chemotherapy. Background technique [0002] Cancer seriously threatens human life and health. Currently, chemotherapy is still the most commonly used means of treating cancer. However, chemotherapy drugs often have systemic toxicity and cause serious adverse reactions. Therefore, it is particularly important to develop a new dosage form that responds to the tumor-specific environment. With the development of nanotechnology, targeted drug carriers based on nanomaterials provide a new solution for tumor-specific drug delivery. Not only that, compared with traditional drug dosage forms, nano-drug carriers are often able to improve the bioavailability, permeability, and stability of anticancer drugs, which further e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/59A61K41/00A61K31/704A61K33/32A61K49/18A61P35/00
CPCA61K31/704A61K33/32A61K41/0057A61K41/0071A61K49/1857A61K47/59A61K47/6923A61P35/00A61K2300/00
Inventor 张羱何若曦双少敏董川
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com