Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus plantarum capable of producing various broad-spectrum antibacterial metabolites and application thereof

A technology of Lactobacillus plantarum and metabolites, applied in the field of microorganisms, can solve the problems of inactivating pathogenic bacteria, achieve strong inhibitory effect, inhibit mononuclear cell proliferation, and have good application prospects

Active Publication Date: 2019-12-03
SOUTHWEST UNIVERSITY
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, chlorine-containing substances cannot completely and effectively inactivate pathogenic bacteria; on the other hand, chlorine easily reacts with organic matter in the washing liquid to generate carcinogenic and toxic substances such as chloroform and haloacetic acid.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus plantarum capable of producing various broad-spectrum antibacterial metabolites and application thereof
  • Lactobacillus plantarum capable of producing various broad-spectrum antibacterial metabolites and application thereof
  • Lactobacillus plantarum capable of producing various broad-spectrum antibacterial metabolites and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Isolation and identification of lactic acid bacteria

[0032] (1) Isolation of microbial strains

[0033] The pickle water samples were diluted with sterilized saline in a 10-fold dilution gradient. Take 100μL of 10 -2 、10 -3 、10 -4 、10 -5 The diluted kimchi water dilution was spread on the MRS solid medium, and incubated at a constant temperature of 37° C. for 48 hours. A single colony of lactic acid bacteria was picked, and the characteristic colony was further isolated and purified repeatedly by the streak separation method, and the morphological characteristics of each colony were recorded and observed by Gram staining microscope.

[0034] The MRS solid medium is composed of the following components in terms of mass percentage: 2% glucose, 1% peptone, 1% beef extract, 0.5% yeast extract, 0.5% sodium acetate, 0.1% Tween 80, dihydrogen citrate 0.1% of amine, 0.02% of magnesium sulfate, 0.005% of manganese sulfate, 1.5% of agar powder, and the balan...

Embodiment 2

[0043] Embodiment 2: the antibacterial activity of plantarum lactobacillus MS031

[0044] (1) Antibacterial activity of bacteria

[0045] The antibacterial activity of the bacteria was determined by the double-layer streak method. Inoculate Lactobacillus plantarum MS031 into MRS liquid medium and cultivate to OD 600nm = 0.8, use an inoculation loop to streak the bacterial suspension onto an MRS agar plate (2 parallel lines, about 2 cm in length), incubate at a constant temperature of 37°C for 12 hours, then add 10 mL of 10 6 CFU / mL LB agar medium (0.7wt % agar) was poured onto the surface of the MRS agar plate, incubated at a constant temperature of 37°C for 24h, and the size of the inhibition zone was observed.

[0046] The result is as figure 2 As shown, Lactobacillus plantarum MS031 showed a strong inhibition zone for the three Gram-positive pathogenic bacteria and four Gram-negative pathogenic bacteria measured, and the antibacterial activity was roughly as follows: E...

Embodiment 3

[0054] Example 3: Inhibitory effect of Lactobacillus plantarum MS031 broad-spectrum antibacterial metabolite mixture on various food-borne pathogens in fresh-cut fruits

[0055] The method described in Example 2 was used to prepare the surface-adsorbed metabolites of Lactobacillus plantarum MS031 and the metabolites secreted into the fermentation broth, mix the two, and decolorize with resin to obtain the broad-spectrum antibacterial metabolites of Lactobacillus plantarum MS031 Mixtures were tested for their ability to control various pathogenic bacteria in fresh-cut fruits as follows:

[0056] (1) Purchase fresh fruit in a local supermarket and cut into pieces, weigh 500g and put them into nine 2L beakers;

[0057] (2) adjusting the concentration of Lactobacillus plantarum MS031 broad-spectrum antibacterial metabolite mixture to 1MIC concentration;

[0058] (3) Culture to OD 600nm =0.1 Listeria monocytogenes, Salmonella and Escherichia coli were resuspended in PBS buffer to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Lactobacillus plantarum MS031 with a deposit number of CGMCC No. 17711. The bacterium has broad-spectrum antibacterial activity and can further produce various antibacterial active metabolites, including bacteriocins capable of inhibiting Gram-positive bacteria and broad-spectrum antibacterial metabolites capable of inhibiting both Gram-positive bacteria and Gram-negative bacteria, and can be used for preparing antibacterial or / and antiseptic products. The broad-spectrum antibacterial metabolites are respectively adsorbed on surface of the bacteria and secreted into fermentation liquor, can effectively inhibit growth of various food-borne pathogenic bacteria such as Listeria monocytogenes, salmonella, escherichia coli and the like in fresh-cut fruits and vegetables,can make up for shortcomings that most existing antibacterial agents have no effect on Gram-negative bacteria, and have good application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a plant lactobacillus producing multiple broad-spectrum antibacterial metabolites and its application. Background technique [0002] Fresh-cut fruits, especially fruit platters and fruit salads, are becoming more and more popular because of their convenience, deliciousness, freshness, nutrition, safety and health, and the market has grown to more than one billion US dollars year by year. In my country, although the fresh-cut fruit industry started late, in recent years, with the improvement of people's living standards, the accelerated pace of life, and the rapid development of the cold chain system, catering industry and retail industry, the market share of fresh-cut fruit is also increasing. rise quickly. However, some research suggests that fresh-cut fruit may be associated with a higher risk of foodborne illness than fresh fruit. Thus, as consumption of fresh-cut fruits...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P21/00A23B7/155C12R1/25
CPCA23B7/155C12N1/20C12P21/00C12N1/205C12R2001/25
Inventor 易兰花祁腾曾凯芳邓丽莉阮长晴
Owner SOUTHWEST UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com