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Preparation method of orange-red fluorescent copper nanoclusters based on l-methionine

A technology of nano-clusters and methionine, which is applied in the fields of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials, and can solve problems such as synthesis and application reports that have not yet been reported, small Stokes shifts, and limited product applications. , to achieve the effect of low detection cost, high selectivity and good fluorescence stability

Active Publication Date: 2022-05-03
HUAIBEI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

L-methionine, also known as methionine, is the only sulfur-containing essential amino acid among the amino acids required for animal growth. It plays an important role in animals. However, using the amino acid molecule L-methionine as a protective agent, The synthesis and application of orange-red fluorescent copper nanoclusters with large Stokes shift have not been reported yet
There is only one related study, that is, our research group reported in 2016 that a copper nanocluster with blue-green fluorescence was synthesized by using L-methionine as a template and a reducing agent by heating in a water bath. However, its synthesis The temperature needs to be controlled at 90°C during the process, and the Stokes shift of the product is small, which limits the further application of the product, and related research is still very challenging.

Method used

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  • Preparation method of orange-red fluorescent copper nanoclusters based on l-methionine
  • Preparation method of orange-red fluorescent copper nanoclusters based on l-methionine
  • Preparation method of orange-red fluorescent copper nanoclusters based on l-methionine

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Experimental program
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Embodiment 1

[0040] Preparation method of orange-red fluorescent copper nanoclusters based on L-methionine:

[0041] Using L-methionine as protective agent, hydrogen peroxide as activator, and hydrazine hydrate as reducing agent, a new method for preparing orange-red fluorescent copper nanoclusters (Cu NCs / Met), the reaction schematic diagram is as follows figure 1 Shown:

[0042] (1) Accurately weigh copper sulfate, dissolve it in ultrapure water, and prepare a concentration of 10 mmol L -1 soluble copper salt solution; accurately weigh L-methionine, with 0.4 mol L -1 NaOH aqueous solution was dissolved to prepare a concentration of 15 mg·mL -1 L-methionine solution; take a certain amount of mass fraction as 30% hydrogen peroxide aqueous solution, dilute it with ultrapure water, and obtain a concentration of 4 mol L -1 The activator, stand-by;

[0043] (2) Measure 3 mL of L-methionine solution in step (1) and place it in a clean reaction glass tube, add 0.9 mL of soluble copper salt ...

Embodiment 2

[0049] Effect of copper sulfate concentration on synthesis:

[0050] (1) Accurately weigh solid copper sulfate, dissolve it in ultrapure water, and prepare copper sulfate aqueous solutions with different concentrations; accurately weigh L-methionine, and use 0.4 mol L -1 Dissolve NaOH to prepare L-methionine solution with a concentration of 15 mg / mL; take hydrogen peroxide and dilute to 4 mol L with ultrapure water -1 ,stand-by;

[0051] (2) Take several clean large test tubes, add the same amount of methionine solution, and add 2 mmol·L dropwise to them under constant stirring. -1 , 5 mmol·L -1 , 10 mmol·L -1 , 15 mmol·L -1 , 20 mmol·L -1 , 25 mmol·L -1 Copper sulfate solution, stirred for ten minutes to mix the two evenly;

[0052] (3) Add 1.5 mL of hydrogen peroxide to the mixed solution obtained in step (2), stir and react for ten minutes, then add hydrazine hydrate to the mixed solution, stir and react at room temperature for 5 hours, and measure the spectrum with...

Embodiment 3

[0054] Effect of L-methionine concentration on synthesis:

[0055] (1) Accurately weigh the copper sulfate solid, dissolve it in ultrapure water, and prepare a concentration of 10 mmol L -1 copper sulfate aqueous solution; accurately weigh L-methionine, and use 0.4 mol·L -1 Dissolve NaOH to prepare methionine solutions with different concentrations; take hydrogen peroxide and dilute to 4 mol L with ultrapure water -1 ,stand-by;

[0056] (2) Take several clean large test tubes and add 5 mg·mL -1 , 10 mg·mL -1 , 15mg·mL -1 , 20mg·mL -1 , 25mg·mL -1 , 30mg·mL -1 Equal volume of L-methionine solution, under constant stirring, add dropwise an equal amount of copper sulfate solution therein, and stir for ten minutes to make the two evenly mixed;

[0057] (3) Add 1.5 mL of hydrogen peroxide to the mixed solution obtained in step (2), stir and react for ten minutes, then add hydrazine hydrate to the mixed solution, stir and react at room temperature for 5 hours, and measure t...

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Abstract

The invention provides a method for preparing orange-red fluorescent copper nanoclusters based on L-methionine. In the method, L-methionine is used as a protective agent, copper salt is used as a metal precursor, and hydrogen peroxide is added as a protective agent. The activator, hydrazine hydrate as the reducing agent, prepared orange-red fluorescent copper nanoclusters (Cu NCs) with good stability. The maximum excitation and maximum emission wavelengths were 337 and 592nm, respectively, and the Stokes shift was as high as 255nm. The structure was recorded as Cu NCs / Met. The synthesis method is novel, economical and easy. According to the fluorescence quenching phenomenon, Cu NCs / Met can be used as a fluorescent nanoprobe for the highly sensitive and selective detection of norfloxacin drug, and the detection process is simple and fast. The fluorescent nanoclusters prepared by this method have great application prospects in the fields of sensing, catalysis, photoelectric devices, anti-counterfeiting, cell imaging, and drug delivery.

Description

technical field [0001] The invention belongs to the field of preparation and application of fluorescent metal nanoclusters, and specifically relates to a new method for preparing L-methionine-based orange-red fluorescent copper nanoclusters and its analysis application. Background technique [0002] Metal nanoclusters (NCs) refer to relatively stable aggregates composed of several to hundreds of metal atoms (Au, Ag, Pt, Pd, Cu, etc.) as the core under the protection of a certain molecular shell. Its size is ultra-small, the particle size is generally less than 2 nm, close to the Fermi wavelength of electrons, and has discrete electronic energy level states and molecular-like properties. As a new type of fluorescent nanomaterial, due to its good biocompatibility , unique electronic, optical and chemical properties, etc., have attracted extensive attention from the research community, and have shown attractive application prospects in the fields of intracellular fluorescence i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B22F1/054C09K11/58G01N21/64
CPCB22F9/24C09K11/58G01N21/6428G01N2021/6432B22F1/0553B22F1/07B22F1/054
Inventor 邵从英李春波李勇倪铮刘献虎
Owner HUAIBEI NORMAL UNIVERSITY
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