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Application of prostaglandin E1 in preparing medicines for treating cerebral hemorrhage

A prostaglandin and drug technology, applied in the field of biomedicine, can solve the problems of no reports, etc., and achieve the effect of reducing the reduction of cell viability, the activation inhibition of microglia cells, and the inhibition of oxidative stress response

Pending Publication Date: 2019-11-29
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, prostaglandin E1 is mainly used clinically for myocardial infarction, thromboangiitis, obliterative arteriosclerosis and other diseases, but what kind of effect prostaglandin E1 also has, then there is no report

Method used

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  • Application of prostaglandin E1 in preparing medicines for treating cerebral hemorrhage
  • Application of prostaglandin E1 in preparing medicines for treating cerebral hemorrhage
  • Application of prostaglandin E1 in preparing medicines for treating cerebral hemorrhage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Application of PGE1 in the treatment of patients with cerebral hemorrhage

[0042] 1. Patients: Between November 2007 and January 2009, 40 patients with hypertensive cerebral hemorrhage were admitted to the Department of Neurology, Affiliated Hospital of Nantong University.

[0043] 2. Inclusion and exclusion criteria: Carry out this embodiment according to the diagnostic criteria formulated by the 1995 National Academic Conference on Cerebrovascular Diseases. CT examination showed that the amount of hemorrhage in the basal ganglia was 10-30ml. The patient is onset for the first time, without any history of chronic liver disease or bleeding disorder, and the platelet measurement is greater than 100×10 9 / L, and the coagulation function was normal. Patients with previous history of intraventricular hemorrhage, subarachnoid hemorrhage and cerebral hemorrhage caused by systemic diseases, as well as severe heart, liver and kidney system dysfunction must be excluded from p...

Embodiment 2

[0074] Application of PGE1 in the treatment of cerebral hemorrhage in mice

[0075] 1. Animals: male C57BL / 6 mice, SPF grade, 26-30g, purchased from the Experimental Animal Center of Nantong University, and normally kept in a clean animal room.

[0076] 2. Experimental grouping: mice were randomly divided into sham operation+NaCl group, ICH group+NaCl group, ICH+PGE1 (2 μg / kg) group, ICH+PGE1 (10 μg / kg), ICH+PGE1 (20 μg / kg), total 5 groups, 30 rats in each group.

[0077] 3. Drug treatment: All mice were intraperitoneally administered (normal saline or PGE1) four times at 0h, 4h, 12h and 24h after operation; all mice received nearly equal liquid injection volume, about 0.5-0.6ml.

[0078] 4. Experimental method: Injecting autologous blood into the brain is a common animal modeling method for cerebral hemorrhage, and this embodiment is used for the therapeutic effect of PGE1 on cerebral hemorrhage. The mouse ICH model was prepared by injecting autologous blood into the brain,...

Embodiment 3

[0104] In this example, for the protection of nerve cells and the inhibition of oxidative stress, heme was used to induce damage to primary cortical neurons to construct an in vitro model of cerebral hemorrhage.

[0105] Construction of heme-induced neuronal apoptosis model (in vitro ICH model): culture mouse cortex neuron cells at embryonic day 15, stimulate with different concentrations of hemin (Normal, DMSO, 1, 10, 50, 100 μM), and select the best stimulating concentration. The result is as Figure 13 As shown, the results of CCK-8 showed that as the hemin concentration increased, the cell viability gradually decreased (1μM hemin cell viability was 0.93±0.19, 10μM hemin cell viability was 0.63±0.15, 50μM hemin cell viability was 0.45±0.09, 100μM hemin cell viability is 0.25±0.04). 50 μM hemin was chosen to stimulate the primary cortical neurons, and the cell viability at different time points was detected. After 18 hours of hemin stimulation, the cell viability was 0.67±...

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Abstract

The invention provides an application of prostaglandin E1 in preparing medicines for treating cerebral hemorrhage, and belongs to the technical field of biological medicines. Compared with the prior art, the application has the advantages that when prostaglandin E1 (PGE1) is used for treating cerebral hemorrhage, compared with the effect of a NaCl blank control treatment group, the prognostic function of cerebral hemorrhage patients is obviously improved; recovery of exercise ability and improvement of nerve functions of cerebral hemorrhage mice are obviously improved; the number of apoptoticnerve cells in tissues around cerebral hematoma of the cerebral hemorrhage mice is obviously reduced; the number of survival nerve cells is obviously increased; and proliferation of astrocytes, activation of microglia and oxidative stress reactions are obviously inhibited. Meanwhile, PGE1 can reduce decrease of cell activity, release of lactic dehydrogenase (LDH) and apoptosis of neurons in in-vitro experiments.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to the application of prostaglandin E1 in the preparation of medicaments for cerebral hemorrhage. Background technique [0002] Intracerebral hemorrhage (ICH) refers to cerebral parenchymal hemorrhage caused by spontaneous rupture of non-traumatic blood vessels in the brain. ICH is a common disease in neurology. Its onset is acute and serious. Although it has been studied for many years, the treatment methods are limited so far. The fatality rate in the acute phase is 30% to 40%. Most of the survivors have different degrees of motor impairment and cognitive impairment. , speech and swallowing disorders and other sequelae. The high mortality rate and high disability rate of this disease bring heavy misery and burden to families and society. With the aging of the population, the harm caused by ICH is becoming more and more serious. [0003] At present, the treatment of cerebral ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/5575A61P7/02A61P9/10
CPCA61K31/5575A61P7/02A61P9/10
Inventor 柯开富沈加兵曹茂红梁晶晶
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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