Biological three-dimensional printing preparation method of anti-inflammatory meniscus stent loaded with cartilage cells

A chondrocyte and three-dimensional printing technology, applied in 3D printing, additive manufacturing, manufacturing tools, etc., to achieve the effect of facilitating exchange and promoting regeneration

Pending Publication Date: 2019-11-22
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, there are no reports or applications of meniscal scaffolds that can have anti-inflammatory effects

Method used

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  • Biological three-dimensional printing preparation method of anti-inflammatory meniscus stent loaded with cartilage cells
  • Biological three-dimensional printing preparation method of anti-inflammatory meniscus stent loaded with cartilage cells
  • Biological three-dimensional printing preparation method of anti-inflammatory meniscus stent loaded with cartilage cells

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0068] As a technical optimization solution of the present invention, a method for preparing three-dimensional bioprinting of an anti-inflammatory meniscus stent loaded with chondrocytes. The method for preparing three-dimensional bioprinting of an anti-inflammatory meniscus stent loaded with chondrocytes includes the following steps:

[0069] S1. Construct a three-dimensional model of the meniscus:

[0070] S1.1. Data scanning: first use a three-dimensional scanning electron microscope to scan the knee joint of the patient on the healthy side, and then store the raw data obtained from the scan into the database;

[0071] S1.2. Data processing: The knee joint model of the patient’s healthy side stored in step S1.1 is segmented and edited by the bio-three-dimensional printer software to obtain the three-dimensional model of the outer or inner side of the knee joint meniscus, and then By mirroring the three-dimensional model of the meniscus, a three-dimensional model of the meniscus su...

Embodiment 1

[0098] 1. Perform nano-level 3D scanning electron microscopy on the left healthy knee joint of the patient, scan the original data, and store it in the database; segment and edit the knee joint image in the biological 3D printer software to obtain the outer side of the meniscus between the left knee joint Three-dimensional model. It is mirrored to obtain a three-dimensional model suitable for the outer side of the patient's right meniscus. Import the 3D data into the biological 3D printer software, perform partial correction and curved surface processing in turn, and save it as a stl format file.

[0099] 2. The processed three-dimensional meniscus model is sliced ​​and layered, and the printed polycaprolactone material and the hydrogel material loaded with fullerene and chondrocytes are respectively set into two printing paths. The two printing paths intersect layer by layer.

[0100] 3. After mixing the collagen and sodium alginate, add them to the DMEM medium, stir to dissolve...

Embodiment 2

[0104] The chondrocyte-loaded anti-inflammatory meniscus scaffold prepared by using the three-dimensional bioprinting technology in Example 1 above was used to characterize the cell survival rate. The scaffold was placed in a 12-well plate for culture, and then the chondrocyte viability in the scaffold was detected by the MTT method. After culturing for 1, 3, 5, 7 and 9 days, discard the old medium and add PBS to rinse to remove dead cells. Add 500 μL of serum-free medium and 1500 μL of MTT solution to each well and incubate at 37°C for 4 hours. Then add 500μL of DMSO to each well to fully dissolve the formazan crystals, take 100μL of the dissolving solution and use a microplate reader to detect the absorbance at a wavelength of 492nm. Three parallel hole experiments were performed at each time point.

[0105] MTT results like Figure 5 It is shown that the chondrocytes on the scaffold have good proliferation behavior and their cell viability is good.

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Abstract

The invention relates to the field of meniscus stents prepared by carrying out biological three-dimensional printing, and specifically discloses a biological three-dimensional printing preparation method of an anti-inflammatory meniscus stent loaded with cartilage cells. The biological three-dimensional printing preparation method of the anti-inflammatory meniscus stent loaded with the cartilage cells is capable of very well imitating three-dimensional macromorphology, internal micro-collagen fiber and porous structure of meniscus tissue, thereby facilitating cell growth and nutrient exchangeso as to promote regeneration of the meniscus tissue. The frame structure of the meniscus stent is printed by adopting selected polycaprolactone; and thus, sufficient mechanical support for the jointis provided. In addition, being loaded with the cartilage cells, the anti-inflammatory meniscus stent is capable of avoiding cell culture operation in vitro after printing; and moreover, the stent isloaded with a fullerene material, so that the stent has anti-inflammatory effects.

Description

Technical field [0001] The invention relates to the field of biological three-dimensional printing meniscus stents, in particular to a biological three-dimensional printing preparation method of anti-inflammatory meniscus stents loaded with chondrocytes. Background technique [0002] Nowadays, meniscus injury is a very common disease. In the United States, more than 1 million people receive meniscus surgery every year. The meniscus is a cartilage tissue, and lack of blood supply results in poor self-healing ability. For severely damaged meniscus, the clinical treatment of meniscus is usually used. Although meniscus resection has the advantages of good short-term curative effect and fast functional recovery, the quality of the regenerated meniscus after meniscus resection cannot perform the biomechanical function of normal meniscus, and it will inevitably aggravate articular cartilage degeneration in the long term. Allogeneic meniscus transplantation can replace the missing men...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/24A61L27/38A61L27/56A61F2/38A61F2/30B33Y10/00
CPCA61L27/56A61L27/24A61L27/20A61L27/3817A61L27/3852A61F2/3872A61F2/30942A61F2/30756B33Y10/00A61L2430/06A61F2002/30985A61F2002/30943A61F2002/30766C08L5/04
Inventor 乔之光孙彬彬戴尅戎刘永佳倪兵王友
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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