A method to improve the survival rate of juvenile abalone during bait transfer period
A technology of survival rate and bait transfer, which is applied in the field of industrial aquaculture, can solve the problems of high mortality, poor feeding, mechanical damage, etc., and achieve the effect of simple operation, cost control and low cost
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Embodiment 1
[0022] Preparation of XS1412C strain fermentation broth:
[0023] Pick a single colony to inoculate the 2216E culture solution, shake and culture at 26°C for 6-8 hours, inoculate the fermentation culture solution with 3% inoculation amount, and ferment at 30°C for 12-24 hours, and the bacterial concentration reaches 1×10 3 -1×10 8 cfu / mL is sufficient.
[0024] Preparation of chitosan-oligosaccharide solution: 10g chitosan was dissolved in 100mL 0.5% acetic acid solution; 35g low-viscosity sodium alginate was dissolved in 500mL seawater, and 30mL 1×10 7 -1×10 8 cfu / mL XS1412C strain fermentation broth, 35 ° C fermentation and enzymatic hydrolysis for 6 hours, boiling water bath inactivation for 10 minutes, after cooling to obtain fucoidan oligosaccharide solution; oligochitosan solution and fucoidan oligosaccharide solution were mixed at a ratio of 1:5, then filtered Sea water to 2L, spare.
[0025] A method for improving the survival rate of juvenile abalone during bait t...
Embodiment 2
[0030] Preparation of XS1412C strain fermentation broth:
[0031] Pick a single colony to inoculate the 2216E culture solution, shake and culture at 26°C for 6-8 hours, inoculate the fermentation culture solution with 4% inoculation amount, and ferment and culture at 30°C for 12-20 hours, until the bacterial concentration reaches 1×10 3 -1×10 8 cfu / mL is sufficient.
[0032] Preparation of chitosan-oligosaccharide solution: 16g chitosan was dissolved in 100mL 0.5% acetic acid solution; 21g low-viscosity sodium alginate was dissolved in 500mL seawater, and 25mL 1×10 7 -1×10 8 cfu / mL XS1412C strain fermentation broth, 35 ° C fermentation and enzymatic hydrolysis for 5 hours, boiling water bath inactivation for 10 minutes, after cooling, a fucoidan oligosaccharide solution was obtained; the chitosan oligosaccharide solution and the fucoidan oligosaccharide solution were mixed at a ratio of 1:5, and then filtered Sea water to 2L, spare.
[0033] A method for improving the surv...
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