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Method for inhibiting and removing biofilm

A biofilm and composition technology, which is applied in sanitary equipment for toilets, disinfection, medical science and other directions, can solve the problems of inability to produce therapeutic effects, treatment failures, and enlargement, and achieves resistance to resistance, efficient destruction, and effective Effects of biofilm inhibition and clearance

Pending Publication Date: 2019-11-15
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, conventional physical and chemical methods (chemical flushing, ultrasonic cleaning) cannot produce effective therapeutic effects on biofilms generated in narrow spaces, and the treatment is more challenging.
Therefore, the treatment of biofilm infection has become the most difficult clinical problem, and with the continuous increase of bacterial resistance, the risk of treatment failure continues to increase

Method used

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  • Method for inhibiting and removing biofilm
  • Method for inhibiting and removing biofilm

Examples

Experimental program
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Effect test

Embodiment 1

[0016] The invention provides a phospholipid microbubble suspension, and the particle diameter of the microbubble is 2.4±0.5um. The gas contained in the microbubble is perfluoropropane; the solvent of the microbubble suspension is physiological saline; the antibacterial agent is vancomycin.

[0017] The inhibition and removal method of the root canal biofilm are as follows:

[0018] 1) Inject physiological saline (control group) and phospholipid microbubble suspension into the root canal of tooth 1 infected by biofilm, the concentration of microbubble is 1*10 7 / ml, 5*10 7 / ml, 1*10 8 / ml, use a syringe to repeatedly pipette 20 times to ensure that the phospholipid microbubbles are evenly distributed in the root canal.

[0019] 2) Put the root canal ultrasonic treatment instrument 3 into the root canal

[0020] 3) Apply ultrasonic treatment, set the ultrasonic frequency to 42 kHz, and the ultrasonic time to 1 min.

[0021] 4) Then rinse the root canal with normal saline t...

Embodiment 2

[0024] The invention provides a surfactant microbubble suspension, and the particle diameter of the microbubble is 1.8±0.4um. The gas contained in the microbubble is air. The solvent of the microbubble suspension is physiological saline; the antibacterial agent is hydrogen peroxide with a mass fraction of 3%.

[0025] The method for inhibiting and removing biofilm on the surface of the medical device is as follows:

[0026] 1) Place the medical device (surgical forceps) in the bracket, and then inject the surfactant microbubble suspension into the bracket, so that the microbubble suspension completely covers the medical device, and finally ensure that the final concentration of the microbubble is 1* 10 6 / ml, and then stirred for 2 minutes to ensure full contact between microbubbles and medical devices.

[0027] 2) Put the bracket into an ultrasonic cleaner for 10 minutes.

[0028] 3) Then use distilled water to rinse the medical device 3 times to wash away the fragments o...

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Abstract

The invention discloses a method for inhibiting and removing a biofilm, which belong to the field of sterilization. The method adopts a cavitation effect to achieve the inhibition and removal of the biofilm, and specifically provides a microbubble suspension comprising microbubbles, an antibacterial agent and a solvent, the microbubble suspension is contacted with the biofilm growing on the surface of a substrate, so that the microbubble suspension completely covers the biofilm, and then a cavitation technology is applied to the microbubble suspension and the biofilm to perform the inhibitionand removal of the biofilm on the surface of a substrate. The method provides an experimental basis for the application of microbubbles in the inhibition and removal of the biofilm, especially the removal of the biofilm in a narrow space, and provides a theoretical basis for the preparation of the highly efficient and safe biofilm removal preparations.

Description

technical field [0001] The invention belongs to the field of physical sterilization, and in particular relates to a method for inhibiting and removing biofilms. Background technique [0002] Biofilm refers to a group of bacteria with special structure and organization that adheres to the surface of the substrate. It can secrete polysaccharide matrix, lipoprotein, etc. on the surface to form hierarchical and ordered extracellular polymeric substances (EPS) to form to protect the biofilm. In real life, many substrates (living tissues, medical devices, engineering materials) will produce biofilms, which seriously threaten people's health. Studies have shown that about 80% of all medical infections come from the biofilm of pathogens, and it is also an important reason for the emergence of drug resistance after bacterial infection. [0003] Biofilms and free bacteria have significantly different characteristics, such as morphological structure, pathogenicity, toxicity, suscepti...

Claims

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Application Information

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IPC IPC(8): A61L2/00A61L2/025A61L2/16A61C19/00
CPCA61L2/00A61L2/025A61L2/16A61C19/002
Inventor 董飞宏李应龙党杰潘洁张珏
Owner PEKING UNIV
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