Application of rumen bypass melatonin in improvement of ruminant rumen microbial flora structure and improvement of milk quality

A technology for rumen microorganisms and ruminants, applied in applications, animal feeds, drug combinations, etc., can solve the problem of no significant effect on milk melatonin content, and achieve the goal of improving milk quality, reducing lactose content, and improving protein utilization. Effect

Active Publication Date: 2019-11-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And feeding rumen melatonin can also affect the hormone concentration in the blood of dairy cows related to immune stress, and feeding rumen melatonin can increase the blood melatonin concentration of dairy cows, but due to the existence of the blood-milk barrier, the Melatonin content in milk did not significantly affect

Method used

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  • Application of rumen bypass melatonin in improvement of ruminant rumen microbial flora structure and improvement of milk quality
  • Application of rumen bypass melatonin in improvement of ruminant rumen microbial flora structure and improvement of milk quality
  • Application of rumen bypass melatonin in improvement of ruminant rumen microbial flora structure and improvement of milk quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Verification of the effect of passing rumen melatonin in reducing somatic cells in milk and improving milk quality

[0077] 1. Feeding of rumen melatonin

[0078] A total of 35 dairy cows with higher somatic cells (300,000 to 1,000,000 / mL somatic cells) were selected and divided into 3 groups. There was no significant difference in body weight within and between groups, with an average body weight of 600 kg. Feed rumen melatonin, wherein the active ingredient melatonin is 40mg group 15 (experimental group 1, that is, each cow has been fed rumen melatonin, wherein the effective dose of melatonin is 40mg), 80mg group 15 cows (experimental group 2, that is, each cow has been fed with ruminal melatonin, and the effective dose of melatonin is 80 mg), and 5 cows in the control group (not fed with rumen melatonin). Continuously fed rumen melatonin for 21 days, collected milk samples every two days, randomly eliminated 5 cows in each experimental group every 7 days, ...

Embodiment 2

[0090] Example 2 Verification of the effect of melatonin on improving the microbial flora in the rumen

[0091] 1. Experimental method

[0092] (1) Put 0.6μg / kg~0.13mg / kg ruminal melatonin into the rumen fistula of 3 rumen fistula cows with similar breeds, ages, and physiological conditions (all the values ​​here refer to the effective dose of melatonin), and 6 It was taken out after 1 hour, and the time when melatonin was injected was recorded as 0 o'clock. Take rumen fluid at 0 o'clock, 6 o'clock and 6 hours after taking out the melatonin preparation (that is, 12 o'clock).

[0093] (2) Extract the rumen microbial flora DNA from the rumen fluid taken at different time points, and then obtain 1,351,116 raw reads through 16SrRNA sequencing. After removing chimeras and short sequences, 1,334,733 effective reads were obtained. 1,334,733 reads, ranging from 25,820 to 316,522.

[0094] 2. Experimental results

[0095] (1) OUT division was performed at the level of sequence simi...

Embodiment 3

[0097] Embodiment 3 Melatonin is on the influence of related hormones in cow's blood and milk

[0098] 1. Feed through the rumen with melatonin

[0099] The feeding method is the same as that in Example 1, and the blood sample is collected at the same time as the milk sample, and blood is collected from the tail vein of the dairy cow.

[0100] 2. Determination of relevant hormone concentrations in serum and milk

[0101] The types of hormones detected in dairy cow serum are: MT, TNF-α, IL-6, IL-10, cortisol. The hormones detected in milk are: MT, TNF-α, IL-10. The assay method is enzyme-linked immunoassay (ELISA).

[0102] 3. Experimental results

[0103] (1) Increase the concentration of melatonin in serum. pass Figure 20 , the melatonin content in the serum of the rumen melatonin 80mg group fed for 7 days was significantly higher than that of the control group (P Figure 21 , the melatonin content in the serum of the rumen melatonin 40mg and 80mg groups fed for 14 days...

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Abstract

The invention provides application of rumen bypass melatonin in improvement of the milk quality and improvement of a ruminant rumen microbial flora structure. According to the application, it is discovered that after melatonin is prepared into the rumen bypass melatonin, a ruminant is orally fed with the rumen bypass melatonin, the somatic cell number of cows is lowered, the somatic cell number ofthe cows can be lowered to 200-400 thousand / mL from 400-700 thousand / mL, the content of milk protein, milk fat and dry substances in milk is obviously increased, the content of lactose in the milk islowered, the milk quality is obviously improved, and in addition, according to the application, it is also discovered that the residual rumen bypass melatonin in the rumen can also improve the abundance of flora in the rumen.

Description

technical field [0001] The invention relates to the technical field of animal nutrition and feed, in particular to the application of rumen-passing melatonin in improving rumen microbial flora structure and milk quality of ruminants. Background technique [0002] With the increase of people's demand for dairy products, the dairy industry is gradually growing, and there are more and more large-scale dairy farms. However, with the expansion of the scale of cattle farms, the problems of dairy cow breeding have gradually become prominent, such as: the reproductive health of dairy cows, the nutritional regulation of dairy cows, etc. Among them, the most harmful and difficult problem to solve is the problem of high somatic cell count in dairy cows. The high or low somatic cell count of dairy cows reflects the health of the udder and is closely related to milk production. Since the somatic cell count of dairy cows brings huge economic losses to dairy farming enterprises every year,...

Claims

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Application Information

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IPC IPC(8): A23K50/10A23K20/132A23K20/158A23K20/20A23K20/163A61K31/4045A61P1/00
CPCA23K50/10A23K20/132A23K20/158A23K20/20A23K20/163A61K31/4045A61P1/00
Inventor 刘国世张鲁姚松阳吴昊杨海路永强郭江鹏王天坤任康杨宇泽
Owner CHINA AGRI UNIV
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