Biosensor for detecting ampicillin, and preparation method and application thereof
A biosensor, ampicillin technology, applied in the field of biosensors, can solve the problems of cumbersome sample pretreatment or concentration process, difficult to popularize, time-consuming and laborious, etc., and achieve the effects of short detection time, low detection limit, and low process cost.
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Embodiment 1
[0064] The preparation method of described biosensor comprises the following steps:
[0065] (1) Preparation of gold nanoparticles;
[0066] (2) Modification of Walkker (containing -SH) and Track (containing -SH) to the surface of gold nanoparticles;
[0067] (3) Mixing the labeled nano-gold solution with the homogeneous reaction solution;
[0068] (4) Hybridization chain reaction, fluorescence detection.
[0069] The preparation method, preferably the preparation steps of gold nanoparticles are as follows:
[0070] (1) After installing the required instruments, add 200ml of ultrapure water into the three-necked flask (be careful not to let dust fall into the three-necked flask).
[0071] (2) Take 500μL (0.04g / ml) HAuCl 4 Put 500μl and 200ml ultrapure water into a single packaged centrifuge tube with a pipette gun, stir and heat at a stirring speed of about 450 rpm until boiling.
[0072] (3) Under the condition of stirring, take 3ml of 1% trisodium citrate solution and q...
Embodiment 2
[0090] A preparation method of the fluorescent biosensor of the present invention:
[0091] The preparation method of described biosensor comprises the following steps:
[0092] (1) Preparation of gold nanoparticles;
[0093] (2) Modification of Walkker (containing -SH) and Track (containing -SH) to the surface of gold nanoparticles;
[0094] (3) Mix the labeled gold nanoparticles solution with the homogeneous reaction solution.
[0095] (4) Hybrid chain reaction, fluorescence detection
[0096] The preparation method, preferably the preparation steps of gold nanoparticles are as follows:
[0097] (1) After installing the required instruments, add 200ml of ultrapure water into the three-necked flask (be careful not to let dust fall into the three-necked flask).
[0098] (2) Take 500μL (0.04g / ml) HAuCl4 in a single-package centrifuge tube, use a pipette gun to take 500μl and 200ml ultrapure water, stir and heat at a stirring speed of about 450 rpm, until boiled.
[0099] (...
Embodiment 3
[0117] The preparation method of described biosensor comprises the following steps:
[0118] (1) Preparation of gold nanoparticles;
[0119] (2) Modification of Walkker (containing -SH) and Track (containing -SH) to the surface of gold nanoparticles;
[0120] (3) Mix the labeled gold nanoparticles solution with the homogeneous reaction solution.
[0121] (4) Hybrid chain reaction, fluorescence detection
[0122] The preparation method, preferably the preparation steps of gold nanoparticles are as follows:
[0123] (1) After installing the required instruments, add 200ml of ultrapure water into the three-necked flask (be careful not to let dust fall into the three-necked flask).
[0124] (2) Take 500μL (0.04g / ml) HAuCl4 in a single-package centrifuge tube, use a pipette gun to take 500μl and 200ml ultrapure water, stir and heat at a stirring speed of about 450 rpm, until boiled.
[0125] (3) Under stirring conditions, take 3ml of 1% trisodium citrate solution and quickly ad...
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