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Bone repair material and preparation method thereof

A bone repair and hydrogel technology, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of particles that cannot be infinitely combined with hydrogels, limited release of magnesium ions, and limited magnesium ions.

Active Publication Date: 2019-11-12
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, micro-nano core-shell particles are difficult to be directly applied to bone defects as bone repair materials, and they need to be compounded into other material systems, which will inevitably affect the release of magnesium ions
Nanocompositehydrogels stabilized by self-assembled multivalent bisphosphonate-magnesium nanoparticles mediate sustained release of magnesium ion and promote in-situ regeneration, Acta Biomaterialia.2017,64:389-400 Introducing phosphate groups into the gel and compounding magnesium salts into the hydrogel can also achieve the purpose of slow release of magnesium ions. Although this method can regulate the release of magnesium ions, the process of forming nanoparticles between magnesium ions and phosphoric acid is mainly through phosphoric acid. Combined with magnesium ions, but the magnesium ions combined by this method are limited, and the formed particles cannot be combined with the hydrogel indefinitely, so that the final release of magnesium ions is limited, and it is difficult to achieve continuous and stable release of magnesium ions in the whole process of bone defect repair. Purpose

Method used

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  • Bone repair material and preparation method thereof
  • Bone repair material and preparation method thereof
  • Bone repair material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A bone repair material, the preparation method is as follows:

[0048] (1) Weigh 9g of chitosan with a molecular weight greater than 20kDa and add it to 300mL of 2% acetic acid aqueous solution. After it is completely dissolved, add 3.3mL of methacrylic anhydride (MA) dropwise to chitosan at a rate of 0.5mL / min. Sugar acetic acid solution, stirred overnight at room temperature, then added 100mL deionized water to the solution and continued to stir to dilute it; put the diluted solution in a 12-14kDa dialysis bag, and dialyze in deionized water at room temperature for 5 days, Change the deionized water every morning and evening, and keep stirring; after dialysis, freeze-dry to obtain white loose methacrylated chitosan.

[0049] (2) Take 1 g of methacrylated chitosan obtained in step (1), dissolve it in 100 mL of deionized water, add 0.05 M MES (monohydrate, about 10.6 g / L) after dissolving, and then add 0.7 g 3-phosphonohexanoic acid (3-phosphonohexanoic acid), after be...

Embodiment 2

[0052] A bone repair material, the preparation method is as follows:

[0053] (1) Weigh 9g of hyaluronic acid with a molecular weight greater than 20kDa and add it to 300mL of deionized aqueous solution. After it is completely dissolved, add 5mL of methacrylic anhydride (MA) dropwise to the aqueous solution of hyaluronic acid at a rate of 0.5mL / min , and add a small amount of sodium hydroxide to the solution, adjust the pH of the solution to weak alkaline, stir overnight under ice bath, then add 100mL deionized water to the solution and keep stirring to dilute it; place the diluted solution in In a 12-14kDa dialysis bag, dialyze in deionized water at room temperature for 5 days, change the deionized water every morning and evening, and keep stirring; after dialysis, freeze-dry to obtain white loose methacrylated hyaluronic acid.

[0054] (2) Take 1 g of the methacrylated hyaluronic acid obtained in step (1), dissolve it in 100 mL of deionized water, add 0.05 M MES (monohydrate...

Embodiment 3

[0057] A bone repair material, the preparation method is as follows:

[0058] (1) Weigh 9g of gelatin with a molecular weight greater than 20kDa and add it to 300mL of deionized aqueous solution. After it is completely dissolved, add 5mL of methacrylic anhydride (MA) dropwise to the gelatin aqueous solution at a rate of 0.5mL / min. Stir overnight, then add 100mL of deionized water to the solution and continue to stir to dilute it; place the diluted solution in a 12-14kDa dialysis bag, dialyze in deionized water at room temperature for 5 days, and change the deionized water every morning and evening water with continuous stirring; after dialysis, freeze-dry to obtain white loose methacrylated gelatin.

[0059] (2) Take 1 g of the methacrylated gelatin obtained in step (1), dissolve it in 100 mL of deionized water, then add 0.7 g of 3-phosphonohexanoic acid, and add 0.3 g of it after it is completely dissolved EDC (1-Ethyl-3-(3'-dimethylaminopropyl)carbodiimide), after stirring ...

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Abstract

The invention provides a bone repair material and a preparation method thereof. The material comprises hydrogel and magnesium-containing micro-nano particles. The mass percentage of magnesium is 0.1-15% based on the total mass of the bone repair material of 100%. The bone repair material provided by the invention can realize the purpose of regulating and controlling the release rate of magnesium ions by regulating and controlling the crosslinking density of hydrogel and the content of groups interacting with magnesium ions. Meanwhile, the bone repair material provided by the invention has large magnesium ion release amount and long magnesium ion release time.

Description

technical field [0001] The invention belongs to the technical field of bone repair materials, and relates to a bone repair material and a preparation method thereof. Background technique [0002] Magnesium metal is a potential bone repair material for clinical use; Magnesium is an essential element in the human body and participates in various important metabolic processes in the human body. More than half of the magnesium in the human body is stored in the bone. Magnesium ions can promote bone formation by up-regulating the expression of calcitonin gene-related polypeptide CGRP in the periosteum. Appropriate concentration of magnesium ions can promote early angiogenesis in bone defects, up-regulate the expression of vascular endothelial growth factor, and promote the expression of middle and late osteogenic genes and bone marrow stem cells. Osteogenic differentiation can promote the proliferation of neural stem cells and the growth of neurite, showing comprehensive therapeu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/04A61L27/20A61L27/22A61L27/50A61L27/52
CPCA61L27/047A61L27/20A61L27/222A61L27/50A61L27/52A61L2300/102A61L2300/412A61L2300/602A61L2430/02C08L5/08C08L5/04
Inventor 赖毓霄陈英奇龙晶秦岭
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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