Application of benzopyran compounds in preparation of anti-hantaan virus (HTNV) drug
A technology for benzopyran and Hantaan virus, which is applied in the application field of benzopyran compounds for preparing anti-Hantaan virus drugs, and achieves the effects of strong inhibitory effect and good in vitro activity
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Embodiment 1
[0015] Embodiment 1: the synthetic route and the method that comprise compound 6 of the present invention
[0016]
[0017] R=H, 2-NO 2 , 4-NO 2 , 2,4-2NO 2 , 3-Br, 3-CF 3 , 2,6-2Cl, 5-CN, 4-CN.
[0018] resolve resolution:
[0019] 10mmol benzaldehyde, 10mmol 3,5-cyclohexanedione or 1,1-dimethyl-3,5-cyclohexanedione, 10mmol malononitrile, 1mmol dimethylaminopyridine and 10mmol benzaldehyde ( or 2-nitrobenzaldehyde, 4-nitrobenzaldehyde, 2,4-dinitrobenzaldehyde, 3-bromobenzaldehyde, 3-fluoromethylbenzaldehyde, 2,6-dichlorobenzaldehyde, 5- cyanobenzaldehyde, 4-cyanobenzaldehyde) was refluxed in 100 milliliters of ethanol for 2-3 hours, and after the reaction was completed, it was cooled to room temperature. After filtering the precipitate, the compound was successively washed with ice ethanol and dried under vacuum.
[0020] Chemical structure identification
[0021] Utilize nuclear magnetic resonance spectrum (NMR) to carry out identification such as molecular weight...
Embodiment 2
[0049] Example 2: Anti-Hantan virus activity detection of compound 1-9 in vitro
[0050] 1 material
[0051] 1.1 Experimental strains and cell lines
[0052] As shown in Table 1, Hantaan virus (HTNV) 76-118 strains infected A549 cells for in vitro drug screening experiments. Both the HTNV 76-118 strain (hereinafter referred to as the virus) and the A549 cell strain were provided by the Department of Microbiology, School of Basic Medicine, Air Force Military Medical University.
[0053] Table 1 Virus strains and cell strains
[0054]
[0055] 1.2 Experimental reagents and consumables
[0056] 1.2.1 Reagents and instruments related to cell culture
[0057] DMEM medium was purchased from Hyclone Company; fetal bovine serum was purchased from Shanghai Sangon Bioengineering Co., Ltd.; 0.25% trypsin was purchased from Hyclone Company.
[0058] The Countstart automatic cell counter was purchased from Shanghai Ruiyu Biotechnology Co., Ltd.; the ultracentrifuge was purchased fr...
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