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Use of thrombin inhibitor in preparation of anti-tumor invasion and metastasis drug

A thrombin inhibitor and anti-tumor technology, which is applied in the field of thrombin inhibitors, divalent direct thrombin inhibitors, preparation of anti-tumor invasion and metastasis drugs, and can solve problems such as tumor deterioration

Inactive Publication Date: 2019-11-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have reported that the occurrence and development of tumors have a bidirectional relationship with blood hypercoagulability, that is, tumor tissue can produce coagulation factors, triggering blood hypercoagulation; and the formation of thrombus can promote tumor deterioration and form a vicious circle; although this Multifaceted relationship involves diverse mechanisms, but researchers suggest anticoagulant drugs could be an attractive anticancer therapy

Method used

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  • Use of thrombin inhibitor in preparation of anti-tumor invasion and metastasis drug
  • Use of thrombin inhibitor in preparation of anti-tumor invasion and metastasis drug
  • Use of thrombin inhibitor in preparation of anti-tumor invasion and metastasis drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Animal experiment

[0036] Reagents used include:

[0037]

[0038]

[0039] The main instruments include:

[0040]

[0041] Methods include:

[0042] Cell growth test: I. Take a 96-well plate and add 200 μL of 1×10 5 / mL cell suspension; II. Dosing according to the ratio, thrombin 10U / mL, r-hirudin 50μg / mL, DTIP 50μg / mL; III. Use 200μL serum-free medium in the blank well as a control; IV. After 24h, The supernatant was discarded, and 100 μL of MTT solution was added to each well, and placed in a 37° C. incubator for 4 hours; V. was measured with a wavelength of 490 nm.

[0043] Cell scratch (Wound Healing assay): I. Place the Culture-Insert 2 Well into a 24-well plate; II. Add 70 μL of 5×105 / mL cells to the two culture chambers, and culture in a 37°C incubator for one day; III. Gently take out the Culture-Insert 2 Well component with tweezers; IV. Add serum-free medium and treat with different drugs, observe and take pictures after 12 hours. ...

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Abstract

The invention belongs to the field of biotechnology, and relates to a novel medicinal use of a thrombin inhibitor, in particular to a use of the thrombin inhibitor, especially a bivalent direct thrombin inhibitor in the preparation of an anti-tumor invasion and metastasis drug. The direct thrombin inhibitor short peptide DTIP with anticoagulant activity is adopted to carry out an in vitro cell test and an in vivo animal experiment, and a test result shows that the bivalent direct thrombin inhibitor DTIP can inhibit tumor invasion and metastasis in vitro; the in vivo experiment shows that the bivalent direct thrombin inhibitor DTIP has an impact on tumorigenesis and development; and further, the direct thrombin inhibitor DTIP can be used for preparing the anti-tumor invasion and metastasisdrug.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a new medicinal application of a thrombin inhibitor, in particular to the application of a thrombin inhibitor, especially a divalent direct thrombin inhibitor, in the preparation of anti-tumor invasion and metastasis drugs. Background technique [0002] Data show that the incidence of malignant tumors remains high, seriously threatening human health, and has had a huge impact on people's lives. According to statistics, both lung cancer and breast cancer in my country have a high incidence rate and high mortality rate; taking lung cancer as an example, the average survival period of patients diagnosed with TNM stage II or above is no more than 48 months. As the level increases, the survival time also decreases. The diagnosis and treatment of malignant tumors such as lung cancer is one of the current research hotspots, among which the research on the prevention and treatment of l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/58A61P35/00A61P35/04
CPCA61K38/58A61P35/00A61P35/04
Inventor 莫炜赵冰于敏张艳玲
Owner FUDAN UNIV
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