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Application of cordate houttuynia extract as adenylate-activated protein kinase activator in preparing medicine for improving metabolic syndrome

A technology of Houttuynia cordata extract and activation of protein kinase, which is applied in the fields of biology and medicine to prevent liver lipid accumulation, good application prospects, and prevent cardiac dysfunction

Inactive Publication Date: 2019-11-05
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that some of the above-mentioned single components such as Houttuynia cordata flavonoids and alkaloids have anti-inflammatory and antibacterial effects respectively, whether there is a synergistic effect between the various components has yet to be elucidated, and it is urgent to develop and maintain their activity to the maximum extent. extraction method
In addition, in addition to exerting the curative effect of clearing away heat and detoxification through anti-inflammatory and antibacterial effects, whether Houttuynia cordata can regulate AMPK as a target to improve the occurrence of metabolic syndrome has not been reported yet.

Method used

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  • Application of cordate houttuynia extract as adenylate-activated protein kinase activator in preparing medicine for improving metabolic syndrome
  • Application of cordate houttuynia extract as adenylate-activated protein kinase activator in preparing medicine for improving metabolic syndrome
  • Application of cordate houttuynia extract as adenylate-activated protein kinase activator in preparing medicine for improving metabolic syndrome

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Experimental program
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Effect test

preparation example Construction

[0027] 2. Preparation of Houttuynia cordata extract

[0028] Wash the fresh Houttuynia cordata, take the above-ground part and put it in a constant temperature drying oven to dry until the weight is constant. After crushing, weigh 10g accurately and put it in a 250mL round bottom flask. Add distilled water according to the ratio of liquid to material 20:1, heat to 90°C and reflux After extracting for 2 hours, stand and soak for 1 hour, filter under reduced pressure, and repeat the above process twice for the filter residue. Combine the filtrates obtained three times, vacuum-dry them, and pass through an 80-mesh sieve to obtain Houttuynia cordata extract, which is a yellow-brown powder in appearance.

[0029] 3. Establishment of animal hyperlipidemia model

[0030] Eight-week-old male C57BL / 6J mice were purchased from Beijing Weitong Lihua Company. After one week of adaptation, the mice were randomly divided into three groups: control group, poloxamer 407 treatment group, and ...

Embodiment 1

[0051] Houttuynia cordata extract has significant blood lipid-lowering effect

[0052] The mice were pretreated with Houttuynia cordata extract at 200 mg / kg / day for 9 days, and the hyperlipidemia model was induced by intraperitoneal injection of poloxamer 407 on the 10th day, and the serum was collected 24 hours later to detect triglyceride, total cholesterol and content of free fatty acids. like figure 1 As shown in A-C, Houttuynia cordata extract can significantly reduce the content of triglyceride, total cholesterol and free fatty acid in serum, and has a good effect of lowering blood lipid.

Embodiment 2

[0054] Houttuynia cordata extract can significantly improve the excessive accumulation of liver lipid

[0055] The mice were pretreated with Houttuynia cordata extract at 200 mg / kg / day for 9 days, and the hyperlipidemia model was induced by intraperitoneal injection of poloxamer 407 on the 10th day, and the mice were sacrificed 24 hours later to take the liver for HE morphological staining , Simultaneously detect the content of liver triglyceride and total cholesterol. like figure 2 A HE staining results showed that Houttuynia cordata extract could significantly improve the excessive accumulation of liver lipid droplets induced by poloxamer 407, and at the same time figure 2 B and 2C show that Houttuynia cordata extract can also significantly reduce the content of triglyceride and total cholesterol in the liver, and has a good effect on inhibiting excessive accumulation of liver lipids related to metabolic disorders.

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Abstract

The invention relates to an application of a cordate houttuynia extract as an adenylate-activated protein kinase activator in preparing a medicine for improving metabolic syndrome. The cordate houttuynia extract is a water-soluble extract of a dried part of a houtuynia sulfite plant, an extraction solvent is distilled water, an extraction ratio is 20:1, and the appearance of the product is yellow-brown powder; The cordate houttuynia extract acts to protect mitochondrial function and anti-oxidation by activating an AMPK-PGC-1alpha / Nrf2 signaling pathway and simultaneously up-regulating mitochondrial production and biphasic enzyme pathways, further improves the metabolic disorders associated with hyperlipidemia, liver lipid accumulation and cardiac dysfunction, and delays the occurrence anddevelopment of metabolic syndrome. The cordate houttuynia extract has no obvious side effects, and is a safe, simple and effective drug.

Description

technical field [0001] The invention belongs to the fields of biology and medicine, and particularly relates to the application of Houttuynia cordata extract as an adenylate-activated protein kinase activator in the preparation of medicines for improving metabolic syndrome. Background technique [0002] Metabolic syndrome is clinically characterized by a series of symptoms of metabolic disorders such as obesity, hypertension, dyslipidemia, impaired glucose tolerance or diabetes, and insulin resistance, and is a pathological state of abnormal aggregation of various metabolic components. With the development of social economy and the change of people's lifestyle, the incidence of metabolic syndrome, which is mainly characterized by metabolic abnormalities such as hypertension, hyperlipidemia, and hyperglycemia, is increasing worldwide, and it is associated with the incidence of cardiovascular and cerebrovascular diseases. The connection is becoming more and more clear, and it ...

Claims

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Application Information

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IPC IPC(8): A61K36/78A61P3/00A61P3/06A61P1/16A61P9/00A61P39/06
CPCA61K36/78A61P1/16A61P3/00A61P3/06A61P9/00A61P39/06
Inventor 刘健康曹可吕伟强冯智辉刘建书
Owner XI AN JIAOTONG UNIV
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