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Serum-free adipose-derived stem cell culture medium and culture method of adipose-derived stem cells (ADSCs)

A technique for adipose stem cells and a culture method, which is applied in the field of serum-free adipose stem cell culture medium and adipose stem cell culture, can solve the problems of insufficient growth-promoting components, damage to the proliferation activity of adipose stem cells, and difficulty in obtaining the number of cells, so as to promote growth and Proliferation ability, reducing proliferative activity damage, and improving the effect of in vitro amplification efficiency

Pending Publication Date: 2019-10-25
安徽科门生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nutrients and growth-promoting components contained in the serum-free medium are insufficient, and the serum-free medium lacks the natural components in the serum to neutralize toxins and protect cells, resulting in damage to the proliferation activity of adipose-derived stem cells
Due to the combined influence of these factors, the proliferation rate of adipose-derived stem cells in serum-free medium is slow, and it is difficult to obtain a sufficient number of cells for clinical use, which cannot meet the needs of clinical applications.

Method used

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  • Serum-free adipose-derived stem cell culture medium and culture method of adipose-derived stem cells (ADSCs)

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Embodiment 1

[0023] A serum-free adipose stem cell culture medium, including: DMEM / F12 medium, transferrin with a final concentration of 8μg / mL, fibroblast growth factor with a final concentration of 30ng / mL, and C- with a final concentration of 2μg / mL Phycocyanin, acanthopanax saponins B with a final concentration of 0.9 μg / mL, cyanidin-3-O-glucoside with a final concentration of 0.8 μg / mL, and β-ecdysterone with a final concentration of 10 μg / mL .

Embodiment 2

[0025] A serum-free adipose stem cell culture medium, including: DMEM / F12 medium, transferrin with a final concentration of 10μg / mL, fibroblast growth factor with a final concentration of 38ng / mL, and C with a final concentration of 2.8ng / mL -Phycocyanin, acanthopanax senticosus B with a final concentration of 1.2μg / mL, cyanidin-3-O-glucoside with a final concentration of 1.6μg / mL, and β-ecdysteroid with a final concentration of 14μg / mL ketone.

Embodiment 3

[0027] A serum-free adipose stem cell culture medium, including: DMEM / F12 medium, transferrin with a final concentration of 12μg / mL, fibroblast growth factor with a final concentration of 40ng / mL, and C- with a final concentration of μg / mL Phycocyanin, acanthopanax saponins B with a final concentration of 1.6μg / mL, cyanidin-3-O-glucoside with a final concentration of 2.2μg / mL, and β-ecdysterone with a final concentration of 20μg / mL .

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Abstract

The invention discloses a serum-free adipose-derived stem cell culture medium and a culture method of adipose-derived stem cells (ADSCs). The culture medium comprises a basic culture medium body and additives, the basic culture base body is a DMEM (dulbecco's modified eagle medium) / F12, the additives include transferrin, fibroblasts growth factors, C-algocyan, acanthopanax senticosus saponins B, scabiolide-3-O-glucosides and beta-ecdysterone. By the arrangement, the growth and proliferation of the ADSCs can be promoted, the damage of proliferation activity of the ADSCs during culture can be reduced, and thus, the in-vitro amplification efficiency of the ADSCs is greatly improved.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a serum-free adipose stem cell culture medium and a method for culturing adipose stem cells. Background technique [0002] Adipose-derived stem cells (ADSCs) are adult stem cells extracted from adipose tissue, which have the potential to differentiate into cartilage, bone, fat and other tissues. Adipose tissue is easily obtained through liposuction or surgery, is less traumatic to the donor and does not involve ethical issues. Moreover, adipose stem cells are relatively widely distributed in humans and animals, and the expansion operation is simple and convenient. They are one of the most potential seed cells in the fields of medicine and tissue engineering. [0003] At present, the in vitro culture of adipose stem cells mainly uses a medium containing fetal bovine serum. Due to the presence of animal-derived serum components, there is a potential safety hazard to cause rejection, and the ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2500/90C12N2501/113C12N2501/115C12N2500/24C12N2500/30C12N2501/998
Inventor 张正亮
Owner 安徽科门生物科技有限公司
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