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Application of leontopodium nivalle extract

An alpine edelweiss, extract technology, applied in the directions of medical preparations containing active ingredients, cosmetic preparations, preparations for skin care, etc., can solve the problem of lack of basement membrane-related gene expression ability, etc. Effect

Pending Publication Date: 2019-10-22
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide an application of alpine edelweiss extract in view of the lack of substances in the prior art that can improve the expression ability of basement membrane-related genes and can be used to stabilize the skin structure

Method used

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  • Application of leontopodium nivalle extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The production of embodiment 1 alpine edelweiss extract

[0031] Alpine edelweiss extract is selected from commercially available products, and can also be prepared by itself according to the existing technology.

[0032] Take 10g of dried alpine edelweiss whole herb, grind it to about 20 meshes with a small medicinal material grinder (Yili 4liang traditional Chinese medicine grinder), and use 10 times the volume of 70% ethanol 500W ultrasonic extraction at room temperature for 2 times, each time for 30min, Centrifuge (8000rpm, 10min) to filter, combine the filtrates, and evaporate the filtrates to dryness to obtain about 1.4g of the edelweiss extract.

Embodiment 2

[0033] The preparation of embodiment 2 skin models

[0034] Extraction of primary human fibroblasts (NHDF) and primary human keratinocytes (NHEK) from skin tissue (extraction method reference: Wu Jinjin, Zhu Tangyou. Skin Tissue Engineering [M]. Beijing: People's Military Medical Publishing House, 2009 :72-103). The primary human fibroblasts were resuscitated. The resuscitation process was as follows: the frozen tube was taken out of the liquid nitrogen, quickly put into a 37°C water bath to melt, and centrifuged at 1000rpm / min for 10min. The recovered cells were cultured with DMEM medium in a carbon dioxide incubator at 37°C for one week, and then digested (take the cells out of the incubator, discard the original medium, add 15 mL of PBS to wash the cells once, add 8 mL of 0.05% pancreatic After protease, incubate in a carbon dioxide incubator at 37°C for 8 minutes, take it out, add 10mL DMEM medium containing FBS to the ultra-clean bench, take out the cells in a 50mL centr...

Embodiment 3

[0035] The test of embodiment 3 skin model

[0036] Use the edelweiss extract evaporated powder prepared in Example 1 and use Hyclone DMEM medium as a solvent to prepare an edelweiss extract solution with a final concentration of 1 mg / mL, and add it when the skin model prepared in Example 2 is cultivated for 3 days Extract solution (the final concentration of Alpine edelweiss extract is 10 μg / ml), and the model without extract solution is used as a pair (NT), and the RNA in the skin model of the test group and the control group is extracted after 18 days for detection.

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Abstract

The invention discloses an application of a leontopodium nivalle extract, and in particular, relates to an application of the leontopodium nivalle extract in preparation of an up-regulating agent forexpression of a col4al gene in human skin or a stabilizer for a structure of a human skin basement membrane. The leontopodium nivalle extract is applied in preparation of the up-regulating agent for expression of the col4al gene in the human skin or the stabilizer for the structure of the human skin basement membrane, and plays roles in regulating expression of the col4al gene and stabilizing thestructure of the human skin basement membrane; in particular, when the leontopodium nivalle extract concentration of the up-regulating agent for expression of the col4al gene in the human skin or thestabilizer for the structure of the human skin basement membrane is 8 [mu]g / ml, the expression of the col4a1 gene, related to the basement membrane stability, in the skin model is up-regulated by 1.54times, so the leontopodium nivalle extract can promote the stability of the structure of the basement membrane, thereby achieving the anti-aging effect.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to an application of alpine edelweiss extract. Background technique [0002] Alpine edelweiss (Leontopodium alpinum) - also known as edelweiss. It is a plant of Compositae, edelweiss. Also known as alpine thin snow grass, small white flowers. It is a famous alpine flower in Europe. [0003] The main application values ​​of alpine edelweiss including its extracts, especially alcohol extracts, include clinical application and cosmetic efficacy. In particular, cosmetic effects have been studied a lot in recent years, but they mainly focus on the effects of whitening, soothing, calming and moisturizing (CN 107106478A, CN 105411900A). [0004] The skin covers the entire human body and is in direct contact with the external environment. In addition to protecting the body from various external factors, the skin is also a mirror that reflects the health of the whole body. Skin is composed of e...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/08
CPCA61K8/9789A61Q19/08A61K2800/82
Inventor 姜丹宋肖洁吕元娣戴竞蒋耀权章漳吴越
Owner JALA GROUP CORPORATION
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