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Lactobacillus plantarum HJ-S2 with functions of reducing cholesterol and enriching selenium and application thereof

A technology of Lactobacillus plantarum, HJ-S2, applied to Lactobacillus, bacteria used in food preparation, application and other directions, can solve problems such as Lactobacillus plantarum that have not yet been found, achieve improved immunity, broad application prospects, good resistance receptive effect

Active Publication Date: 2019-10-11
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has become a research hotspot in recent years to screen out probiotics from samples from different sources and develop them into corresponding health products and foods to regulate human health. Lactobacillus plantarum with two functions of selenium

Method used

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  • Lactobacillus plantarum HJ-S2 with functions of reducing cholesterol and enriching selenium and application thereof
  • Lactobacillus plantarum HJ-S2 with functions of reducing cholesterol and enriching selenium and application thereof
  • Lactobacillus plantarum HJ-S2 with functions of reducing cholesterol and enriching selenium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Isolation and preliminary screening of bacterial strains

[0025] 1. Isolation and purification of bacterial strains

[0026] The lactobacillus plantarum in the present invention is isolated and screened from the intestinal tract of beaked whales living below 300 meters in the deep sea.

[0027] Collect samples from the intestinal tract of beaked whales, take 10 g of samples and grind the grinding liquid in a mortar for 30 minutes on a shaker, and carry out gradient dilution of the grinding liquid in 10 mL sterile saline from 10 -1 to 10 -6 . Draw 200 μL of grinding liquid in the ultra-clean workbench and apply it to MRS-CaCO 3 On the agar plate, culture in a constant temperature incubator at 37°C for 48 hours, select a single colony that produces obvious calcium dissolution circles for multiple purifications, and store the slant for future use.

[0028] 2. Colony morphology observation and catalase experiment

[0029] 2.1 Gram staining: pick the colonie...

Embodiment 2

[0032] Example 2: Functional screening of bacterial strains

[0033] Determination of the cholesterol-lowering ability of strains

[0034] MRS-CHOL medium: MRS medium 54g, cholesterol 1.0g, Tween 20mL, ox bile salt 3.0g. Put 1.0g of cholesterol in 20mL Tween 80 and heat it to boil to dissolve it, then slowly pour it into the culture medium while it is hot, and it will be in the state of micellar solution. The color of the culture medium is opaque light yellow. After the prepared medium was sterilized at 121°C for 20 minutes, shake the test tube while it was still hot or shake it upside down to completely dissolve the jelly, then cool it naturally at room temperature, and store it for later use.

[0035] The purified Lactobacillus colonies were picked, transferred to MRS liquid medium, and activated at 37°C for 24 hours, and the activation was repeated twice. The activated strain was inoculated into the MRS-CHOL medium with 2.0%, and cultured on a shaker at 37°C for 24 hours....

Embodiment 3

[0045] Embodiment three: the identification of bacterial strain

[0046] 1. Colony characteristics and morphology under the microscope

[0047] After culturing Lactobacillus plantarum HJ-S2 on MRS agar medium at 37°C for 48 hours, observe the shape of the strain on the plate. The colony diameter of this strain is 0.5-2mm, the color is milky white, the colony is moist, the edges are neat, and it is a round protrusion. The results are as follows: figure 1 . Gram staining, observe the morphological characteristics of the bacteria under the oil microscope, it is a spore-free Gram-positive bacillus, round-ended straight rod-shaped, single, paired or short-chain, facultative anaerobic, the results are as follows figure 2 .

[0048] 2. Physiology and biochemistry of the strain and API50CHI experiment (Mérieux, France)

[0049] Lactobacillus plantarum HJ-S2 is Gram positive, catalase negative, does not hydrolyze starch, does not liquefy gelatin, does not produce hydrogen sulfide, ...

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Abstract

The invention discloses a lactobacillus plantarum HJ-S2 (Lactobacillus plantarum) with the functions of reducing cholesterol and enriching selenium and application thereof. The strain was preserved inthe General Microbiological Center of China Committee for Culture Collection of Microorganisms on May 7st, 2019, and the preservation number is CGMCC No.17720. The strain is separated from the intestinal viscus of a beaked whale living 300 meters or below the sea level, and the strain has the certain effects of reducing cholesterol and enriching selenium, has certain tolerance to acid and cholates and is very high in safety and capable of being applied to the fields of drugs, health care products, foods, beverages and the like.

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to a marine source Lactobacillus plantarum HJ-S2 (Lactobacillus plantarum) with cholesterol-lowering and selenium-enriching effects and its application. Background technique [0002] In recent years, the incidence of cardiovascular and cerebrovascular diseases such as hypertension, coronary heart disease and arteriosclerosis has been increasing year by year, seriously affecting human health. According to incomplete statistics, about 17.5 million people die of cardiovascular and cerebrovascular diseases in the world every year, about Accounted for 33% of the total number of deaths. Atherosclerosis and the lack of selenium in the body are important causes of cardiovascular and cerebrovascular diseases. At the same time, hyperlipidemia is one of the important risk factors leading to atherosclerosis. Hyperlipidemia refers to abnormal levels of one or several lipids in the bloo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/06A23L2/38A23L33/135C12R1/25
CPCC12N1/20A61K35/747A61P3/06A23L2/382A23L33/135A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/3204A23V2200/324A23V2200/3262
Inventor 唐旭万婧倞徐长安黄仕新吴鹏
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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