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Transintron Molecular Marker of Wheat Powdery Mildew Resistance Gene mlxbd and Its Application

A technology for wheat powdery mildew and powdery mildew resistance genes, which is applied in biochemical equipment and methods, DNA/RNA fragments, and microbial determination/inspection, etc. Accelerates the breeding process with high accuracy

Active Publication Date: 2022-05-27
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The local variety (Triticum.aestivum) has not been widely promoted due to its limited adaptability and its own defects, but it carries many excellent resistance genes

Method used

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  • Transintron Molecular Marker of Wheat Powdery Mildew Resistance Gene mlxbd and Its Application
  • Transintron Molecular Marker of Wheat Powdery Mildew Resistance Gene mlxbd and Its Application
  • Transintron Molecular Marker of Wheat Powdery Mildew Resistance Gene mlxbd and Its Application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Acquisition and identification of separated groups

[0039] With the disease-resistant variety of small white winter wheat and the disease-susceptible variety CC, F was obtained by hybridization 2 Colony.

[0040] Inoculate the wheat powdery mildew bacteria E09 to CC, culture at 16-20 °C to obtain sufficient strains. Inoculation with BgtE09, F of small white winter wheat and CC combination was used by flick method 2 Isolated groups were identified for disease resistance at the seedling stage (1 to 2 leaf stage), and F was to be fully affected by CC in the control group 2 Isolated groups were identified, and the reactive type of wheat powdery mildew was investigated according to the grading criteria of 0 to 4 (Sheng Baoqin, 1988, wheat seedling stage powdery mildew, plant protection, 1, 49), of which type 0 was immune, 0; type 1 was near immune, level 1 was high resistance, level 2 was moderate resistance, level 3 was moderate sensitivity, and level 4 was high sensitivity...

Embodiment 2

[0059] To verify the effect of the molecular labels 7BLCOS-9 and 7BLCOS-8 in the selection of actual breeding, we × CC F from powdery mildew based on the identification results of powdery mildew resistance 2 Homozygous disease-resistant plants, homozygous disease-susceptible plants, and heterozygous susceptible plants were randomly selected in the generation population, and THE CROSS-INTRON PRIMERS 7BLCOS-9 and 7BLCOS-8 were used for PCR amplification verification, with small white winter wheat (containing powdery mildew resistance gene mlxbd) and CC (no powdery mildew resistance gene) as controls, respectively.

[0060] The application of the molecular marker of the present invention in the detection of wheat powdery mildew resistance gene mlxbd, comprising the following specific steps:

[0061] (1) Proceedings of the National Academy of Sciences of the United States of America, 1984, 81:8014-8018;

[0062] (2) The PCR reaction system is: total volume 20 μL, containing 10× Tag Bu...

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Abstract

The present invention relates to the technical field of crop genetics and breeding, a molecular marker for auxiliary selection of wheat powdery mildew resistance gene mlxbd, named 7BLCOS‑9 and 7BLCOS‑8, which have good repeatability and high resolution; Application of molecular markers for wheat powdery mildew resistance gene mlxbd-assisted selection to detect F 2 The DNA of a single plant is used as a template, and the molecular marker primers 7BLCOS-9 or 7BLCOS-8 of the wheat powdery mildew resistance gene mlxbd assisted selection are amplified by PCR, and the fragment size of the molecular marker obtained according to the amplification is used to judge whether in a single plant It contains the powdery mildew resistance gene mlxbd; the application method is a simple, practical, and highly accurate marker-assisted selection method for the powdery mildew resistance gene mlxbd, which can greatly improve the breeding efficiency.

Description

Technical field [0001] The present invention belongs to the field of crop genetic breeding technology, specifically provides a transintron molecular marker for wheat powdery mildew resistance gene mlxbd auxiliary selection and its application. Background [0002] Wheat (Triticum aestivum L.) Powdery mildew is an important wheat disease caused by Powdery mildew. According to the gene-to-gene theory: for each resistance gene of the host plant, sooner or later the pathogen will appear a corresponding toxic gene. Breeding disease-resistant varieties is the most cost-effective way to prevent powdery mildew in wheat. [0003] With the rapid development of molecular markers, there are many types of molecular markers, and different markers have their own advantages and disadvantages, but in general, functionality, polymorphism and versatility are the criteria for measuring the advantages and disadvantages of markers. In breeding work, molecular markers can be used for gene localization, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 薛飞金彦龙胡经煌
Owner SHIHEZI UNIVERSITY
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