Gene sequence modification method based on codon synonymous mutation of codon and its application in vaccine preparation

A technology of gene sequence and synonymous mutation, applied in the direction of plant gene improvement, application, genetic engineering, etc., can solve the problem of virulence reversion, improve fidelity, improve genetic stability, and reduce the probability of virulence reversion. Effect

Inactive Publication Date: 2019-10-08
安子琛
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Problems solved by technology

In animal vaccines, there is also the problem of strong virule...

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  • Gene sequence modification method based on codon synonymous mutation of codon and its application in vaccine preparation
  • Gene sequence modification method based on codon synonymous mutation of codon and its application in vaccine preparation
  • Gene sequence modification method based on codon synonymous mutation of codon and its application in vaccine preparation

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Embodiment 1

[0044] Poliomyelitis is an acute infectious disease caused by poliovirus. The disease spreads widely and is more common in children, hence the name polio. The virus mainly invades the central nervous system, damages the motor nerve cells in the anterior horn of the spinal cord, and causes limb paralysis and paralysis. There are currently no effective drugs to treat polio.

[0045] The disease is mainly prevented and controlled by using vaccines. At present, there are live attenuated vaccines (oral sugar pill vaccines, taken directly or dissolved in cold boiled water; oral liquid vaccines, directly dropped in the baby's mouth or on biscuits), and inactivated vaccines (injection, injected into the mouth). infants). Oral sugar pills: Infants generally take one pill each at the age of 2, 4, and 6 months; and then take another pill at the age of 1.5 to 2, 4, and 7 years old. Oral liquid vaccine: Infants usually start in the second month, 2 drops each time, with an interval of 4...

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Abstract

The invention discloses a gene sequence modification method based on codon synonymous mutation of codon and its application in vaccine preparation. The method is transformed into TTA, TTG, TCA or TCGby base mutation of a synonymous codon in a sequence. in the process of gene duplication, only one base needs to be mutated to form a stop codon (TAA, TGA, TAG), and the probability of termination ofpeptide chain synthesis by gene mutation is greatly increased, and the species or virus genetic stability thereby can be greatly increased. In the use of an attenuated live vaccine for viruses, safetyis the main problem, and the risk of virulence reversion of the attenuated live vaccine is always existed. In the design of the attenuated live vaccine, synonymous mutation of the codon of the viralgene can improve the fidelity of a vaccine sequence, and the risk of virulence reversion of vaccine poisons in the body is reduced, so that the safety of the attenuated live vaccine is increased.

Description

technical field [0001] The invention relates to a gene sequence modification method, in particular to a gene sequence modification method based on codon synonymous mutation, and also relates to the gene sequence obtained by the modification method and its application in vaccine preparation. The invention belongs to the field of biotechnology. Background technique [0002] In a nucleic acid sequence, three consecutive nucleotide residues are a codon. The standard genetic code is made up of 64 codons, and almost all living things use the same genetic code. Even viruses with non-cellular structures use standard genetic codes, 64 codons and their encoded amino acids such as figure 1 shown. [0003] Among the 64 codons, except for 3 stop codons (TAA, TAG, TGA), the remaining 61 codons represent 20 amino acids. Except for methionine and tryptophan, which each have one codon, the other 18 amino acids have two or more codons. Different codons corresponding to the same amino aci...

Claims

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Application Information

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IPC IPC(8): C12N15/41A61K39/13A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2770/32022C12N2770/32334A61K2039/5254
Inventor 安子琛
Owner 安子琛
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