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Novel application of trollius altaicus extract

A technology of lotus flower extract and Altai gold, which is applied in the field of traditional Chinese medicine, can solve the problems of undiscovered Streptococcus mutans and achieve high application value and development prospects

Active Publication Date: 2019-10-08
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no report on Streptococcus mutans has been found in the Altai trompeii

Method used

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  • Novel application of trollius altaicus extract
  • Novel application of trollius altaicus extract
  • Novel application of trollius altaicus extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the preparation of Altay Trollica extract

[0025] Take the dried Altai Tsura chinensis, crush it, add 10 times the amount of 70% ethanol to reflux for extraction for 2 hours, filter it, and freeze-dry the obtained extract for later use. The extracts of Altai troglodytes used in the present invention are the extracts of Altai troglodytes prepared in this example. It should be noted that this example is only an exemplary preparation method of a kind of Altai Tsura chinensis extract, and all the extracts of Altai Tsura chinensis obtained by those skilled in the art through the prior art are all The present invention can be realized.

Embodiment 2

[0026] Embodiment 2, the preparation of streptococcus mutans suspension

[0027] Inoculate the standard strain of Streptococcus mutans (ATCC 700610, purchased from China Common Microorganism Culture Collection Center) that has been resuscitated routinely in BHI liquid medium, culture it in a constant temperature incubator at 37°C for 24 hours, and culture it on a BHI plate for 24 hours. , pick a single colony in BHI liquid medium for pure culture in a 37°C constant temperature incubator; take the bacterial cells in the logarithmic growth phase and adjust the absorbance (OD) to 0.2 at a wavelength of 630nm with a UV spectrophotometer to obtain a concentration of about 10 6 cfu mL -1 Mutans Streptococcus suspension, set aside.

Embodiment 3

[0028] Embodiment 3, antimicrobial susceptibility test of the extract of Altai Trollica chinensis

[0029] After dissolving the Altai Tsura chinensis extract with 10% dimethyl sulfoxide (DMSO) to make a mother solution with a final concentration of 40mg / mL; take the mother solution for dilution, and the diluted concentrations are 20mg / mL, 10mg / mL, 5mg / mL, respectively. mL, 2.5mg / mL, 1.25mg / mL.

[0030] Get 100 μ L of the bacterial suspension prepared in Example 2 and evenly spread it on the BHI flat plate, punch holes; set up multiple groups of experiments, add 20 μ L of medicinal solution in each hole, and the medicinal solutions of the test groups are respectively different concentrations (40, 20 . The BHI plate was cultured in a constant temperature incubator at 37°C for 24 hours, and the diameter of the inhibition zone was measured by the cross method. Three wells were paralleled for each concentration, and the average value was calculated. As a result of the culture, it...

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Abstract

The invention relates to the field of traditional Chinese medicines, in particular to a novel application of a trollius altaicus extract, and especially relates to an application of the trollius altaicus extract to preparation of a streptococcus mutans inhibitor. Experiments show that the trollius altaicus extract has streptococcus mutans inhibiting activity and can inhibit growth of streptococcusmutans in a floating state and inhibit formation of a streptococcus mutans biofilm state. Therefore, the trollius altaicus extract can be used for preparing the streptococcus mutans inhibitor and preparing a medicine for preventing and / or treating caries and an oral care product for improving the caries, and has high application values and development prospects. Moreover, the trollius altaicus extract is made of natural materials and can avoid the side effects of products such as traditional antibiotics and fluoride on human bodies.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to the new application of the Altai troglodytes extract, in particular to the application of the Altai troglodytes extract in the preparation of streptococcus mutans inhibitors. Background technique [0002] Dental caries, commonly known as tooth decay and tooth decay, is a common multifactorial bacterial disease in the oral cavity, which can be secondary to pulpitis and apical periodontitis, and even cause alveolar bone and jaw inflammation. The course of dental caries develops relatively slowly, but its incidence is high and its distribution is wide. The World Health Organization has listed it as one of the three major diseases of human beings, along with tumors and cardiovascular diseases. The currently accepted theory of caries etiology is the quadruple factor theory, which is mainly related to bacteria, cavity environment, host and time. Bacteria are a necessary con...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P1/02A61P31/04A61K8/9789A61Q11/00A61K133/00
CPCA61K36/71A61K2236/333A61K2236/51A61K2800/84A61Q11/00A61K8/9789A61P1/02A61P31/04
Inventor 田树革闫莉周晓英曾亚
Owner XINJIANG MEDICAL UNIV
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