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Nephrotic syndrome progression-related metabolic markers and application thereof

A technology for nephrotic syndrome and metabolic markers, which is applied in the field of screening and identification of nephrotic syndrome markers, and can solve the problem that the pathological process of nephrotic syndrome cannot be determined.

Active Publication Date: 2019-09-27
SHANXI UNIV
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  • Abstract
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Problems solved by technology

[0005] In order to solve the shortcomings of the prior art that the course of nephrotic syndrome cannot be determined, the present invention provides a metabolic marker related to the course of nephrotic syndrome and its application

Method used

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  • Nephrotic syndrome progression-related metabolic markers and application thereof
  • Nephrotic syndrome progression-related metabolic markers and application thereof
  • Nephrotic syndrome progression-related metabolic markers and application thereof

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Embodiment Construction

[0029] Materials and instruments: Dionex UltiMate 3000 ultra-high performance liquid chromatography and quadrupole-electrostatic field orbitrap high-resolution mass spectrometry, (Dionex UltiMate 3000 UHPLC-Q Exactive HR-MS, Thermo-Fisher, USA), Xcalibur workstation (Thermo Fi Oiler Scientific Inc., Waltham, Ma, USA), TGL-16 high-speed desktop refrigerated centrifuge (Hunan Xiangyi Centrifuge Instrument Co., Ltd.); KYJ-3 special air source for nitrogen blowing instrument (Beijing Spot Technology Co., Ltd.) ; Sartorius BSA124S analytical balance (Sartorius, Germany).

[0030] Reagents: HPLC grade acetonitrile, methanol and formic acid (Fisher Scientific); Adriamycin (doxorubicin hydrochloride for injection, Shenzhen Wanle Pharmaceutical Co., Ltd., batch number: 1409E3).

[0031] Animals: SPF grade male SD rats, provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., animal license number SCXK (Beijing) 2013-0014; the animal breeding room was kept at a temper...

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Abstract

The present invention belongs to the technical field of screening and identification of nephrotic syndrome markers, and provides nephrotic syndrome progression-related metabolic markers and application thereof. Bis(4-ethylbenzyl)sorbitol, C16 d-sphinganine, lysophosphatidylethanolamine (LPE) (22:6), lysophosphatidylcholine (LPC) (22:5), N-ethanolamine and proline are taken as progressive markers; oleic acid amide, docosahexaenoic acid, 14S-hydroxy-docosahexaenoic acid, docosapentaenoic acid, uric acid, and LPC (20:5) and (18:1) are taken as early markers; and acylcarnitine (C18:0), linoleic acid carnitine, LPC (18:3), DL-tryptophan, indoleacrylic acid, p-cresol sulfate, 3-indol sulfate, cytosine, creatinine, sphingosine-1-phosphoric acid, L-aleuritic-dihydrosphingosine and ceramide (d18:0 / 14:0) are taken as late markers. The nephrotic syndrome progression-related metabolic markers provided by the present invention can be quantified, are fast, and have high sensitivity and strong specificity.

Description

technical field [0001] The invention belongs to the technical field of screening and identification of nephrotic syndrome markers, and in particular relates to metabolic markers related to nephrotic syndrome lesion process and application thereof. Specifically, two adriamycin nephropathy models and LC-MS technology were used to analyze the markers related to the progress of nephrotic syndrome and their use in the preparation of diagnostic and differential reagents for the progress of nephrotic syndrome. Background technique [0002] Nephrotic syndrome shows the symptoms of massive proteinuria, hypoalbuminemia and hyperlipidemia, which is a syndrome caused by changes in the permeability of the glomerular filtration membrane due to various causes, and nephrotic syndrome is more common in children Refractory diseases occur with a high incidence rate and seriously affect human health. The clinical diagnosis is mainly based on urine protein quantification and plasma albumin leve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N33/487
CPCG01N30/88G01N33/48707G01N2030/8813G01N2800/347
Inventor 秦雪梅杨柳李爱平张王宁张丽增
Owner SHANXI UNIV
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