Enzyme/calcium salt/GO (graphene oxide) functional nanocomposite and preparation method thereof

A nano-composite material and composite material technology, applied in the field of enzyme/calcium salt/graphene oxide functional nano-composite material and its preparation, to achieve the effect of simple equipment and gentle and simple experimental process

Pending Publication Date: 2019-09-27
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, as far as the field of enzyme-nanomaterials is concerned, it is still in the exploratory stage, and the research on combining enzyme-calcium salt-graphene oxide to form composite nanomaterials has not been reported.

Method used

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  • Enzyme/calcium salt/GO (graphene oxide) functional nanocomposite and preparation method thereof
  • Enzyme/calcium salt/GO (graphene oxide) functional nanocomposite and preparation method thereof
  • Enzyme/calcium salt/GO (graphene oxide) functional nanocomposite and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] An enzyme / calcium salt / graphene oxide functional nanocomposite material, comprising the following components in mass percentage: 27.17% tyrosinase, 67.39% calcium phosphate crystal, and 5.43% GO.

[0036] (1) Preparation of materials

[0037] 4 mg GO was ultrasonically dissolved in PBS (0.06 mol / L) with a pH of 8.0, 20 mg tyrosinase and 0.667 mL 240 mM calcium chloride solution were added in sequence, shaken well, and reacted at room temperature for 48 h. Then the precipitate was separated, washed and freeze-dried in a vacuum freeze dryer for 4 h, and then the dried nanocomposite was observed with a scanning electron microscope, and its nanostructure was as follows: figure 1 . Depend on figure 1 It can be seen that the composite nanostructure is a uniform nanoflower cluster composed of 80-120 nm rod-like structures.

[0038] (2) Determination method and results of laccase activity in nanocomposite materials

[0039] Take 3.5 mL of citric acid-disodium hydrogen phosp...

Embodiment 2

[0043] An enzyme / calcium salt / graphene oxide functional nanocomposite material, comprising the following components in mass percentage: laccase 40%, calcium phosphate crystal 32%, GO 28%.

[0044] (1) Preparation method of nanocomposite materials

[0045] 2 mg GO was ultrasonically dissolved in PBS (0.2 mol / L) with a pH of 5.7, 15 mg laccase and 0.667 mL of 120 mM calcium chloride solution were added in turn to shake well, and the reaction was carried out at 25 °C and 140 rpm in a shaker for 48 h. Afterwards, the precipitate was separated, washed and freeze-dried in a vacuum freeze dryer at -30 °C for 6 h.

[0046] (2) Determination method and results of laccase activity in nanocomposite materials

[0047] Take 3.5 mL of citric acid-disodium hydrogen phosphate buffer solution with a pH of 4.6, add 0.5 mL of 2 mM ABTS solution, and then add 1 mL of the sample solution to be tested to start the reaction. After a certain period of time, filter the reaction solution with a filter h...

Embodiment 3

[0053] An enzyme / calcium salt / graphene oxide functional nanocomposite material, comprising the following components in mass percentage: 40% carbonic anhydrase, 32% calcium phosphate crystals, and 28% GO.

[0054] (1) Preparation method of nanocomposite materials

[0055] Dissolve 5 mg GO ultrasonically in PBS (0.2 mol / L) with a pH of 8.5, add 15 mg carbonic anhydrase and 0.667 mL 120 mM calcium acetate solution in turn, shake well, and react in a shaker at 25 °C and 140 rpm for 48 h , and then the precipitate was separated, washed and freeze-dried in a vacuum freeze-dryer at -30 °C for 6 h.

[0056] (2) Determination method and results of carbonic anhydrase activity in nanocomposite materials

[0057] Mix 0.3 mL of the enzyme solution to be tested, 2.4 mL of PBS (pH=7), and 0.3 mL of p-NPA (0.02 M in acetonitrile), and react for a certain period of time to measure the difference in absorbance , to calculate the enzyme activity. Finally, the enzyme activity of carbonic anhyd...

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Abstract

The invention provides an enzyme/calcium salt/GO (graphene oxide) functional nanocomposite and a preparation method thereof. The nanocomposite is prepared from components in percentage by mass as follows: 20%-40% of bio-enzyme, 32%-70% of calcium phosphate crystals and 5%-30% of GO. A dynamic reaction method is adopted, violent experiment methods such as high temperature are not required, no organic reagents are involved, the method conforms to the green and environmental protection route, the obtained enzyme/calcium salt/GO functional nanocomposite has a regular structure, immobilized enzyme activity, thermal stability and storage stability are improved under the condition that enzyme conformation and GO nanostructures are not damaged, and meanwhile, the nanocomposite has certain antibacterial and adsorption performance and can be widely applied to antibacterial composites and adsorption composites.

Description

technical field [0001] The invention relates to the technical field of chemical materials, in particular to an enzyme / calcium salt / graphene oxide functional nanocomposite material and a preparation method thereof. Background technique [0002] Enzyme is a kind of biocatalyst, which has the advantages of strong selectivity, mild reaction conditions and simple process. Traditional enzyme immobilization methods can improve the performance of certain enzymes. With the rapid development of modern society, various ingenious preparation techniques apply different types of materials to enzyme immobilization, and the performance of enzyme immobilization is getting higher and higher. In previous studies, it was found that multi-walled carbon nanotubes were used as a carrier for immobilizing laccase to speed up electron transfer, and the enzyme activity of laccase was maintained at 85.3%; while the use of magnetic ferric oxide nanoparticles to immobilize cellulase, the enzyme activity ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N9/96C12N9/02C12N9/88B01J20/24B01J20/20B01J20/04
CPCC12N11/14C12N9/96C12N9/0061C12Y110/03002C12N9/88C12Y402/01001C12N9/0059C12Y110/03001B01J20/24B01J20/20B01J20/048
Inventor 段林林盛宏林张亚涛米立伟陈卫华
Owner ZHONGYUAN ENGINEERING COLLEGE
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