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Microbial mixed flora capable of promoting plant growth

A technology that promotes plant growth and mixed flora. It is applied in the direction of plant growth regulators, plant growth regulators, microorganisms, etc. It can solve problems affecting the quality of surface water and groundwater, soil compaction and acidification of cultivated land, and decline in the quality of agricultural products. Stable and controllable, strong tolerance and adaptable effect

Active Publication Date: 2019-09-27
LINYI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater

Method used

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  • Microbial mixed flora capable of promoting plant growth
  • Microbial mixed flora capable of promoting plant growth
  • Microbial mixed flora capable of promoting plant growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Microbial mixed flora promotes growth of hydroponic plants

[0018] The specific implementation steps are as follows:

[0019] Flavobacterium pectinovorum CGMCC12362 (denoted as bacteria A), Lysobacter ruishenii CGMCC10136 (denoted as bacteria B), Enterobacter mori CGMCC10322 (denoted as bacteria C), were inoculated separately Into LB medium, cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the hydroponic system, and the culture was continued for 1 week. In the present invention, a blank control group is also set, and...

Embodiment 2

[0025] Microbial mixed flora promotes soil culture plant growth

[0026] The specific implementation steps are as follows:

[0027] Flavobacterium pectinovorum CGMCC12362 (denoted as bacteria A), Lysobacter ruishenii CGMCC10136 (denoted as bacteria B), Enterobacter mori CGMCC10322 (denoted as bacteria C), were inoculated separately Into LB medium, cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the soil culture system, and the culture was continued for 1 week. Under the same conditions, an equal amount of water was added to...

Embodiment 3

[0033] Determination of Auxin Secreting Ability of Microbial Strains

[0034] The specific implementation steps are as follows:

[0035] Flavobacterium pectinovorum CGMCC12362 (denoted as bacteria A), Lysobacter ruishenii CGMCC10136 (denoted as bacteria B), Enterobacter mori CGMCC10322 (denoted as bacteria C), were inoculated separately Into LB medium, cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0. Take 100 mL of the three bacterial cultures respectively, and use high-performance liquid chromatography-tandem mass spectrometry to measure the auxin (IAA) content therein, as shown in Table 3 below.

[0036] Table 3 Results of strain secretion auxin experiment

[0037] Growth hormone secretion capacity (mg / L) Bacteria A 306.5 Bacteria B 356.1 Bacteria C 328.2

[0038] It can be known from Example 3 that all three strains have the ability to secrete auxin, which can promote plant growth and increase crop yield ...

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Abstract

The invention discloses a microbial mixed flora capable of promoting plant growth and belongs to the technical field of agricultural microorganisms. The microbial mixed flora comprises flavobacterium pectinovorum, lysobacter ruishenii and enterobacter mori and is prepared with a method comprising steps as follows: an LB culture medium is inoculated with flavobacterium pectinovorum, lysobacter ruishenii and enterobacter mori, and flavobacterium pectinovorum, lysobacter ruishenii and enterobacter mori are subjected to shaking culture at the temperature of 30 DEG C until the microbial concentration O.D600 is approximately equal to 2.0 and then are mixed in the volume ratio of 1:1:1. The mixed flora has a synergistic effect on the plant growth, is superior to a growth promoting effect of a single strain or pair-wise combined strains and has higher adaptability in the actual soil environment.

Description

technical field [0001] The invention relates to a mixed flora composed of multiple microbial strains, in particular to a method for the flora to promote plant growth, and belongs to the technical field of agricultural microorganisms. Background technique [0002] Promoting yield increase is the primary task of modern high-efficiency agriculture. In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater. Therefore, controlling the amount of chemical fertilizers is a practical need to promote cost savings, energy conservation and emission reduction, and is of great significance to ensure national food...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/20C12R1/01
CPCA01N63/00C12N1/20
Inventor 李强许菡亓文钰韩琪
Owner LINYI UNIVERSITY
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