Stem cell directed differentiation device and method of use thereof

Abstract

The invention discloses a device for directional differentiation of stem cells and a method for using the same, belonging to the field of cell differentiation devices. A device for directional differentiation of stem cells comprises a bacterial culture dish body and a culture cover. A first annular sealing groove is dug at the upper end of the bacterial culture dish body. The lower end of the culture cover is fixedly connected with an integral sealing ring matching the first annular sealing groove. A round hole is dug in the center of the culture cover, and an auxiliary culture cover is arranged at the round hole. rod, and a pair of cylindrical rotating rods are symmetrically distributed on the auxiliary culture cover with the center of the auxiliary culture cover as the center. Partial flipping, compared with the original flipping method, can reduce the area of ​​contamination during the partial flipping process by at least 95%, ensuring the smooth preparation of embryoid bodies and promoting the directional differentiation process of stem cells.

Description

technical field [0001] The present invention relates to the field of cell differentiation devices, and more particularly, to a stem cell directional differentiation device and a method for using the same. Background technique [0002] Heart failure caused by myocardial cell dysfunction is a major disease worldwide, and patients with heart failure ultimately die because of the loss of the heart's pumping function or arrhythmia. One-year mortality in patients with severe heart failure exceeds 50%. Although heart transplants can be performed in advanced patients, about 20% of patients die while waiting for an organ transplant due to a shortage of donor organs. Due to the high morbidity and mortality of heart failure, the death of the transplanted heart, complications such as immune rejection, and the late failure of the transplanted heart, it is urgent to develop new therapeutic methods to improve the function of cardiomyocytes and prevent the occurrence of heart failure. Cur...

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Problems solved by technology

[0015] It is not difficult to find that in the above process of preparing embryoid bodies, after the ES cell suspension is dropped on the lid, the lid is turned over and placed on the upper end of the bacterial culture dish. During the process of dripping and turning, the bacteria in the surrounding air are very easy to stick. Attached to the entire inner surface of the lid, a lid with a large area of ​​contamination on the inner surface can greatly affect the preparation of embryoid bodies, resulting in the failure of directed differentiation

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