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Neural stem cell culture medium and culture method

A technology of neural stem cells and culture methods, applied in the direction of nervous system cells, cell culture active agents, culture process, etc., can solve the problems of slow proliferation, difficult to obtain cell number, complex components, etc. Increase efficiency, promote the effect of clinical research and application

Pending Publication Date: 2019-09-20
安徽科门生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the medium used for neural stem cells generally has the problems of complex components and slow proliferation, and it is difficult to obtain a sufficient number of cells

Method used

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  • Neural stem cell culture medium and culture method

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Effect test

Embodiment 1

[0021] A kind of neural stem cell culture medium, the composition is as follows: DMEM / F12 culture medium, the insulin of final concentration is 5 μ g / mL, the transferrin of final concentration is 10 μ g / mL, the basic fibroblast growth factor that final concentration is 10 ng / mL , epidermal growth factor at a final concentration of 20 ng / mL, lipoic acid at a final concentration of 20 ng / mL, glutathione at a final concentration of 1.5 μg / mL, vitamin C at a final concentration of 3 μg / mL, and a final concentration of 2 μg / mL mL of ginsenoside Rb1, salvianolic acid A at a final concentration of 0.5 μg / mL, and resveratrol at a final concentration of 1 μg / mL.

Embodiment 2

[0023] A kind of neural stem cell culture medium, the composition is as follows: DMEM / F12 culture medium, the insulin of 8 μ g / mL final concentration, the transferrin of 14 μ g / mL final concentration, the basic fibroblast growth factor of 13 ng / mL final concentration , epidermal growth factor at a final concentration of 22ng / mL, lipoic acid at a final concentration of 25ng / mL, glutathione at a final concentration of 2μg / mL, vitamin C at a final concentration of 4μg / mL, and a final concentration of 2.2μg / mL mL of ginsenoside Rb1, salvianolic acid A at a final concentration of 0.8 μg / mL, and resveratrol at a final concentration of 1.2 μg / mL.

Embodiment 3

[0025] A kind of neural stem cell culture medium, the composition is as follows: DMEM / F12 culture medium, the insulin of final concentration is 10 μ g / mL, the transferrin of final concentration is 15 μ g / mL, the basic fibroblast growth factor that final concentration is 15 ng / mL , epidermal growth factor with a final concentration of 30 ng / mL, lipoic acid with a final concentration of 30 ng / mL, glutathione with a final concentration of 2.5 μg / mL, vitamin C with a final concentration of 5 μg / mL, and a final concentration of 3 μg / mL mL of ginsenoside Rb1, salvianolic acid A at a final concentration of 1 μg / mL, and resveratrol at a final concentration of 1.5 μg / mL.

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Abstract

The invention discloses a neural stem cell culture medium and a culture method. The culture medium comprises a basic culture medium and additives, wherein the basic culture medium is a DMEM / F12 culture medium; and the additives comprise insulin, transferrin, basic fibroblast growth factor, epidermal growth factor, lipoic acid, glutathione, vitamin C, ginsenoside Rb1, salvianolic acid A, and resveratrol. The neural stem cell culture medium and the culture method can effectively improve the proliferation speed of neural stem cells and the in vitro amplification efficiency of the neural stem cells, and thus enough neural stem cells are rapidly obtained, so that the neural stem cell culture medium and the culture method are beneficial to promoting clinical research and application of the neural stem cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a neural stem cell culture medium and a culture method. Background technique [0002] Neural stem cells (NSCs) are neural precursor cells with high self-renewal and proliferation capabilities. It has multiple differentiation potentials, and can differentiate into neurons, astrocytes and oligodendrocytes under certain conditions, and has the potential to treat a variety of nervous system diseases, especially in the treatment of neurodegenerative diseases and injuries. It has a good application prospect and has become a research hotspot in the field of neuroscience. In order to promote the clinical research and application of neural stem cells, it is very important to develop a medium suitable for the expansion and culture of neural stem cells in vitro. At present, the medium used for neural stem cells generally has the problems of complex components and slow proliferation, and it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2500/25C12N2501/115C12N2501/11C12N2501/999C12N2500/38C12N2500/30
Inventor 张正亮
Owner 安徽科门生物科技有限公司
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