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A kind of biological preparation and preparation method for preventing and treating yak gastrointestinal diseases

A technology for gastrointestinal diseases and biological preparations is applied in the field of biological preparations and preparations for the prevention and treatment of yak gastrointestinal diseases, and can solve the problems of bacterial infection, complicated preparation process, and limited development of intestinal probiotic preparations.

Active Publication Date: 2020-11-03
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because yaks live in plateaus and alpine regions for a long time, the gastrointestinal tract environment is very different from that of ordinary cattle species. Therefore, the industry has not been able to provide a suitable intestinal probiotic compound preparation
In addition, although the use of intestinal probiotics has many benefits, the preparation process is cumbersome, and it is very susceptible to bacterial infection, which is one of the important reasons for the limited development of intestinal probiotics

Method used

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  • A kind of biological preparation and preparation method for preventing and treating yak gastrointestinal diseases
  • A kind of biological preparation and preparation method for preventing and treating yak gastrointestinal diseases
  • A kind of biological preparation and preparation method for preventing and treating yak gastrointestinal diseases

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Experimental program
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preparation example Construction

[0038] 2. The present invention also provides a preparation method of the biological preparation. Specifically include the following steps:

[0039] 1. Cultivate Bacillus natto, Bacillus bulgaricus, Clostridium butyricum, Lachnospira multipaires, and Eubacterium saccharolyticum using the culture medium respectively, and cultivate them on a constant temperature shaker at 40°C until the amount of viable bacteria in each medium is ≥6×10 8 CFU / ml. According to the mass fraction, the medium components include: 1% beef extract, 2% yeast powder, 5% sucrose, 1% peptone and the rest sterile water.

[0040]Subsequently, each culture medium was centrifuged at 6000-10000 g for 10 min to obtain each bacterial cell pellet. Then according to the ratio of viable bacteria: Bacillus natto: Bacillus bulgaricus: Clostridium butyricum: Lachnospira multipair: Eubacterium saccharolyticus=1:2~5:5~10:3~5:3~5, the The cells of each group were mixed evenly to obtain the mixed cell pellet. Then mix t...

Embodiment 1

[0060] Example 1: Effect of microbial lyoprotectant on biological preparations

[0061] 1. Make 14 groups of biological preparations, each with 9 repetitions. The preparation method is as follows: according to the ratio of live bacteria: Bacillus natto: Bacillus bulgaricus: Clostridium butyricum: Lachnospira multipair: Bacillus saccharolyticum = 1:3:10:3:3, and the bacteria of each group were precipitated Mix evenly to obtain mixed cell pellets. Then add a microbial freeze-drying protective agent whose mass is 5 times the mass of the mixed bacterial cell sedimentation, equilibrate in a water bath at 25°C for 45 minutes, and freeze in liquid nitrogen for 25 seconds to obtain the desired biological preparation.

[0062] Wherein, the microbial lyoprotectants in each group are shown in Table 1. The values ​​in Table 1 are all calculated on the basis of 100ml of base liquid, which is diluted fermentation broth or sterile water. For example, if the base liquid is the fermentation...

Embodiment 2

[0068] Example 2: Effects of microbial composition on intestinal microbial composition and dietary nutrient digestibility of young yaks

[0069] 1. Select 30 6-month-old yaks and divide them into 10 groups, with 3 repetitions in each group, including 9 experimental groups and 1 control group. House feeding under the same conditions for 60 days; each yak was fed twice a day (at 9:00 and 19:00), 0.5 kg of concentrate was fed each time, and water was freely available. The concentrate was purchased from Beijing Zemu Jiuyuan Biotechnology Research Institute. The experimental group was fed 10 g of biological preparations before feeding the concentrate at 9:00. The feeding method was to mix the biological preparations with clean water and then pour them. The feeding frequency was: every 3 days of continuous feeding of biological preparations, stop taking 1 sky. The control group was fed with the same amount of water at the same time. The lyoprotectants of the biological preparatio...

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Abstract

The invention belongs to the field of biological preparations, and in particular relates to a biological preparation for preventing and treating yak gastrointestinal diseases and a preparation method. The specific technical scheme includes: a biological preparation for preventing and treating yak gastrointestinal diseases. According to the amount of viable bacteria, the composition of the biological preparation is: Bacillus natto: Bacillus bulgaricus: Clostridium butyricum: Lachnospira multipair: Puertocoli Eubacterium = 1:2~5:5~10:3~5:3~5. In the biological preparation, the amount of viable bacteria is ≥6×10 8 CFU / ml. The invention also provides a specific preparation method of the biological preparation. Utilizing the biological preparation provided by the invention can help young cattle quickly establish probiotic flora in the gastrointestinal tract, can be used as daily intestinal health food for adult cattle, and can also be used as auxiliary medicine for sick cattle to help sick cattle recover quickly .

Description

technical field [0001] The invention belongs to the field of biological preparations, and in particular relates to a biological preparation for preventing and treating yak gastrointestinal diseases and a preparation method. Background technique [0002] Yak has been the dominant domestic animal in China's plateau area since ancient times, and it is also an important economic source for the survival and development of herdsmen. However, because yaks have been raised mainly by grazing for a long time, the situation of "summer work, autumn fat, winter thinness, and spring death" has occurred in yak breeding, which has caused huge losses to herdsmen. The gastrointestinal tract is an important part of the body to digest and absorb nutrients, and is closely related to the growth performance of yaks. [0003] Supplementing intestinal probiotics is one of the important means to promote the digestive activity of the gastrointestinal tract and improve the absorption capacity of the b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/747A61K9/19A61K47/26A61K47/36A61K47/22A61P1/00A61P1/14A23K50/10A23K10/18C12N1/20A61K35/742A61K35/741C12R1/07C12R1/225C12R1/145C12R1/01
CPCA23K10/18A23K50/10A61K9/19A61K35/741A61K35/742A61K35/747A61K47/22A61K47/26A61K47/36A61P1/00A61P1/14C12N1/20A61K2300/00
Inventor 王利
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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