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Biological agent for preventing yak gastrointestinal diseases and preparation method

A technology for gastrointestinal diseases and biological preparations is applied in the field of biological preparations and preparations for the prevention and treatment of yak gastrointestinal diseases, and can solve the problems of limited development of intestinal probiotic preparations, cumbersome preparation processes, and miscellaneous bacterial infections.

Active Publication Date: 2019-09-20
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because yaks live in plateaus and alpine regions for a long time, the gastrointestinal tract environment is very different from that of ordinary cattle species. Therefore, the industry has not been able to provide a suitable intestinal probiotic compound preparation
In addition, although the use of intestinal probiotics has many benefits, the preparation process is cumbersome, and it is very susceptible to bacterial infection, which is one of the important reasons for the limited development of intestinal probiotics

Method used

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  • Biological agent for preventing yak gastrointestinal diseases and preparation method
  • Biological agent for preventing yak gastrointestinal diseases and preparation method
  • Biological agent for preventing yak gastrointestinal diseases and preparation method

Examples

Experimental program
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Effect test

preparation example Construction

[0038] 2. The present invention also provides a preparation method of the biological preparation. Specifically include the following steps:

[0039] 1. Cultivate Bacillus natto, Bacillus bulgaricus, Clostridium butyricum, Lachnospira multipaires, and Eubacterium saccharolyticum using the culture medium respectively, and cultivate them on a constant temperature shaker at 40°C until the amount of viable bacteria in each medium is ≥6×10 8 CFU / ml. According to the mass fraction, the medium components include: 1% beef extract, 2% yeast powder, 5% sucrose, 1% peptone and the rest sterile water.

[0040]Subsequently, each culture medium was centrifuged at 6000-10000 g for 10 min to obtain each bacterial cell pellet. Then according to the mass ratio: Bacillus natto: Bacillus bulgaricus: Clostridium butyricum: Lachnospira multipair: Bacillus saccharolyticus=1:2~5:5~10:3~5:3~5, each group The cells were mixed evenly to obtain a mixed cell pellet. Then mix the mixed bacterial precipi...

Embodiment 1

[0060] Example 1: Effect of microbial lyoprotectant on biological preparations

[0061] 1. Make 14 groups of biological preparations, each with 9 repetitions. The preparation method is as follows: according to the mass ratio: Bacillus natto: Bacillus bulgaricus: Clostridium butyricum: Lachnospira multipair: Eubacterium saccharolyticum = 1:3:10:3:3, the sedimentation and mixing of each group of bacteria , to obtain a mixed cell pellet. Then add a microbial freeze-drying protective agent whose mass is 5 times the mass of the mixed bacterial cell sedimentation, equilibrate in a water bath at 25°C for 45 minutes, and freeze in liquid nitrogen for 25 seconds to obtain the desired biological preparation.

[0062] Wherein, the microbial lyoprotectants in each group are shown in Table 1. The values ​​in Table 1 are all calculated on the basis of 100ml of base liquid, which is diluted fermentation broth or sterile water. For example, if the base liquid is the fermentation dilution o...

Embodiment 2

[0068] Example 2: Effects of microbial composition on intestinal microbial composition and dietary nutrient digestibility of young yaks

[0069] 1. Select 30 6-month-old yaks and divide them into 10 groups, with 3 repetitions in each group, including 9 experimental groups and 1 control group. House feeding under the same conditions for 60 days; each yak was fed twice a day (at 9:00 and 19:00), 0.5 kg of concentrate was fed each time, and water was freely available. The concentrate was purchased from Beijing Zemu Jiuyuan Biotechnology Research Institute. The experimental group was fed 10 g of biological preparations before feeding the concentrate at 9:00. The feeding method was to mix the biological preparations with clean water and then pour them. The feeding frequency was: every 3 days of continuous feeding of biological preparations, stop taking 1 sky. The control group was fed with the same amount of water at the same time. The lyoprotectants of the biological preparatio...

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Abstract

The invention belongs to the field of biological agents, and particularly relates to a biological agent for preventing yak gastrointestinal diseases and a preparation method. The technical scheme particularly includes that the biological agent for preventing the yak gastrointestinal diseases comprises, by viable bacteria quantity, bacillus natto, Bacillus bulgaricus, clostridium butyricum, Lachnospira multiparus and non-sucroclastic Eubacterium with the ratio of 1:(2-5):(5-10):(3-5):(3-5). The viable bacteria quantity of the biological agent is larger than or equal to 6*10<8>CFU / ml. The invention further provides the particular preparation method of the biological agent. By the aid of the biological agent, young yaks can be helped to rapidly build gastrointestinal probiotic groups, the biological agent can serve as daily intestinal healthcare food for adult yaks and can also be used as adjuvant drugs for diseased yaks, and the diseased yaks are helped to rapidly recover.

Description

technical field [0001] The invention belongs to the field of biological preparations, and in particular relates to a biological preparation for preventing and treating yak gastrointestinal diseases and a preparation method. Background technique [0002] Yak has been the dominant domestic animal in China's plateau area since ancient times, and it is also an important economic source for the survival and development of herdsmen. However, because yaks have been raised mainly by grazing for a long time, the situation of "summer work, autumn fat, winter thinness, and spring death" has occurred in yak breeding, which has caused huge losses to herdsmen. The gastrointestinal tract is an important part of the body to digest and absorb nutrients, and is closely related to the growth performance of yaks. [0003] Supplementing intestinal probiotics is one of the important means to promote the digestive activity of the gastrointestinal tract and improve the absorption capacity of the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K9/19A61K47/26A61K47/36A61K47/22A61P1/00A61P1/14A23K50/10A23K10/18C12N1/20A61K35/742A61K35/741C12R1/07C12R1/225C12R1/145C12R1/01
CPCA23K10/18A23K50/10A61K9/19A61K35/741A61K35/742A61K35/747A61K47/22A61K47/26A61K47/36A61P1/00A61P1/14C12N1/20A61K2300/00
Inventor 王利
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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