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A method for detecting bird's nest by electrochemical fingerprint

A fingerprint and electrochemical technology, which is applied in the field of electrochemical fingerprint detection of bird's nest, can solve the problems of identifying bird's nest, etc.

Active Publication Date: 2022-04-29
ZHONGSHAN FLASHLIGHT POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no research on the use of electrochemical oscillation reaction to determine the electrochemical fingerprint of bird's nest, and there is no report on the use of its fingerprint characteristic parameters to identify bird's nest

Method used

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  • A method for detecting bird's nest by electrochemical fingerprint
  • A method for detecting bird's nest by electrochemical fingerprint
  • A method for detecting bird's nest by electrochemical fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Electrochemical fingerprint of the present invention identifies bird's nest

[0029] 1) Preparation of sample hydrolyzate

[0030] The sample to be tested was dried to constant weight, ground, and passed through a 120-mesh sieve. Add 0.1120g of sample powder and 5mL of 1.0mol / L sulfuric acid to the glass test tube, place it in an oven for hydrolysis at 40°C for 8 hours, filter, and take the filtrate to obtain the sample hydrolyzate;

[0031] 2) B-Z electrochemical oscillation fingerprint

[0032] Take step 1) 5mL sample hydrolyzate, 5mL 2.0mol / L H 2 SO 4 , 10mL 1.8mol / L HOOCCH 2 COOH, 10mL 0.016mol / L (NH 4 ) 4 Ce(SO 4 ) 4 , placed in a 150mL glass reaction vessel, shake well, and cover the reactor cover with injection holes and electrodes. At 35°C, with a stirring speed of 500r / min, add 10mL of 1.0mol / L KBrO 3 , at this time in the mixed solution [H + ]=0.75mol L -1 ,

[0033] [Ce 4+ ]=0.004mol L -1 、[HOOCCH 2 COOH]=0.45mol L -1 , [BrO 3 ...

Embodiment 2

[0035] Embodiment 2 Electrochemical fingerprint of the present invention identifies bird's nest

[0036] 1) Preparation of reference hydrolyzate

[0037] Take the genuine bird's nest and dry it to constant weight, grind it, and pass it through a 120-mesh sieve. Add 0.1120g of bird’s nest powder and 5mL of 1.0mol / L sulfuric acid to the glass test tube, place in an oven for hydrolysis at 40°C for 8 hours, filter, and take the filtrate to obtain bird’s nest hydrolyzate;

[0038] 2) Preparation of sample hydrolyzate

[0039] The sample to be tested was dried to constant weight, ground, and passed through a 120-mesh sieve. Add 0.1120g of sample powder and 5mL of 1.0mol / L sulfuric acid to the glass test tube, place it in an oven for hydrolysis at 40°C for 8 hours, filter, and take the filtrate to obtain the sample hydrolyzate;

[0040] 3) B-Z electrochemical oscillation fingerprint

[0041] Step 1) 5mL bird's nest hydrolyzate, 5mL 2.0mol / L H 2 SO 4 , 10mL 1.8mol / L HOOCCH 2 CO...

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Abstract

The invention provides a method for detecting bird's nest by electrochemical fingerprint spectrum, which adopts B-Z oscillation reaction detection, and the specific operation steps are as follows: (1) preparation of bird's nest hydrolyzate; (2) B-Z electrochemical oscillation fingerprint spectrum; (3) Analysis of electrochemical fingerprints. The method for detecting bird's nest by electrochemical fingerprint of the present invention adopts BrO 3 ‑ ‑Ce 2+ ‑H + ‑The identification of bird's nest by malonic acid oscillation system can obtain characteristic information without complicated pretreatment. It is an accurate, simple and low-cost identification method with good application prospects.

Description

technical field [0001] The invention specifically relates to a method for detecting bird's nest by electrochemical fingerprinting. Background technique [0002] Bird's nest, also known as jelly, is mostly produced in Indonesia, Thailand, the Malay Archipelago, Australia and my country's Hainan Dazhou Island. It is a jelly-like substance spit out by swiftlets, which has high nutritional value, and anti-virus and anti-inflammatory properties. Medicinal value. Bird's nest is generally composed of water, lipids, sialic acid, protein, carbohydrate compounds, amino acids, fibers, inorganic substances, etc. However, different types and sources of bird's nest have different compositions and characteristics. As the market of bird's nest products is expanding day by day, the profits are huge, and the phenomenon of adulteration is becoming more and more serious. Its nutritional components, pharmacological effects, adulteration and identification methods have become current research hot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 李小玉刘敬赵斌李晓璐张娜谢彩玲熊文明
Owner ZHONGSHAN FLASHLIGHT POLYTECHNIC
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